PXD027534 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome of mouse striatal dopamine synaptosomes purified by fluorescence sorting |
Description | Dopamine transmission is a monoaminergic system involved in reward processing and motor control. Volume transmission is thought to be the main mechanism by which monoamines modulate effector transmission though synaptic structures are scarcely described. In the present work we aimed to unravel the cellular and molecular synaptome of single projection pathways (Zhu F, Cizeron M, Qiu Z, Benavides-Piccione R, Kopanitsa MV, Skene NG, Koniaris B, DeFelipe J, Fransén E, Komiyama NH & Grant SGN (2018) Architecture of the Mouse Brain Synaptome. Neuron 99: 781–799.e10). To that end, we established a workflow combining fluorescence tracing of the dopaminergic pathway, fluorescence activated synaptosome sorting and mass spectrometry-based proteomics. With this approach we provide the first ex-vivo model to thoroughly analyse the cellular and molecular organisation of dopaminergic synapses from mouse striatum. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-09 |
AnnouncementXML | Submission_2022-06-09_04:17:39.883.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stephane Claverol |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-25 14:33:21 | ID requested | |
⏵ 1 | 2022-06-09 04:17:40 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Dopamine, synapse, synaptosome, FASS, mouse, striatum, proteome |
Contact List
Stephane Claverol |
contact affiliation | Plateforme Proteome, Univ.Bordeaux |
contact email | stephane.claverol@u-bordeaux.fr |
lab head | |
Stephane Claverol |
contact affiliation | Plateforme Proteome |
contact email | stephane.claverol@u-bordeaux.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027534
- Label: PRIDE project
- Name: Proteome of mouse striatal dopamine synaptosomes purified by fluorescence sorting