PXD027479

PXD027479 is an original dataset announced via ProteomeXchange.

Title	Translational repression of NMD targets by GIGYF2 and EIF4E2
Description	Translation of messenger RNAs (mRNAs) with premature translation termination codons produces truncated proteins with potentially deleterious effects. This is prevented by nonsense-mediated mRNA decay (NMD) of these mRNAs. NMD is triggered by ribosomes terminating upstream of a splice site marked by an exon-junction complex (EJC), but also acts on many mRNAs lacking a splice junction after their termination codon. We developed a genome-wide CRISPR flow cytometry screen to identify regulators of mRNAs with premature termination codons in K562 cells. This screen recovered essentially all core NMD factors and suggested a role for EJC factors in degradation of PTCs without downstream splicing. Among the strongest hits were the translational repressors GIGYF2 and EIF4E2. GIGYF2 and EIF4E2 mediate translational repression but not mRNA decay of a subset of NMD targets and interact with NMD factors genetically and physically. Our results suggest a model wherein recognition of a stop codon as premature can lead to its translational repression through GIGYF2 and EIF4E2.
HostingRepository	PRIDE
AnnounceDate	2021-10-12
AnnouncementXML	Submission_2021-10-12_03:33:00.145.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Niladri Sinha
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-21 07:53:21	ID requested
⏵ 1	2021-10-12 03:33:00	announced

Dataset with its publication pending

submitter keyword: NMD, Translation, GIGYF2, EIF4E2

Rachel Green
contact affiliation	Howard Hughes Medical Institute and Department of Molecular Biology and Genetics, Johns Hopkins Medical Institute, 725 N. Wolfe Street 714 PCTB Baltimore, MD 21205 410-614-4928
contact email	ragreen@jhmi.edu
lab head
Niladri Sinha
contact affiliation	Johns Hopkins Medical Institute
contact email	niladrisinha23@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027479
     1. Label: PRIDE project
     2. Name: Translational repression of NMD targets by GIGYF2 and EIF4E2