PXD027452 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Annexin A1 is a polarity cue that directs planar mitotic spindle orientation during mammalian epithelial morphogenesis |
Description | Oriented cell divisions are critical for the formation and maintenance of structured epithelia. Proper mitotic spindle orientation relies on polarised anchoring of force generators to the cell cortex by the evolutionarily conserved Gαi-LGN-NuMA complex. However, the polarity cues that control cortical patterning of this ternary complex remain largely unknown in mammalian epithelia. Here we identify the membrane-associated protein Annexin A1 (ANXA1) as a novel interactor of LGN in mammary epithelial cells. ANXA1 acts independently of Gαi to instruct the accumulation of LGN and NuMA at the lateral cortex to ensure cortical anchoring of Dynein-Dynactin and astral microtubules and thereby planar alignment of the mitotic spindle. Loss of ANXA1 randomises mitotic spindle orientation, which in turn disrupts epithelial architecture and lumen formation in three-dimensional (3D) primary mammary organoids. Our findings establish ANXA1 as an upstream cortical cue that regulates LGN to direct planar cell divisions during mammalian epithelial morphogenesis. |
HostingRepository | PRIDE |
AnnounceDate | 2022-12-02 |
AnnouncementXML | Submission_2022-12-02_03:52:06.238.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | PaulSkipp |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-20 22:26:38 | ID requested | |
⏵ 1 | 2022-12-02 03:52:06 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mammary,Proteomics, Annexin A1 |
Contact List
SalahElias |
contact affiliation | Biological Sciences University of Southampton |
contact email | s.k.elias@soton.ac.uk |
lab head | |
PaulSkipp |
contact affiliation | Centre for Proteomic Research, University of Southampton |
contact email | pjss@soton.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027452
- Label: PRIDE project
- Name: Annexin A1 is a polarity cue that directs planar mitotic spindle orientation during mammalian epithelial morphogenesis