PXD027373

PXD027373 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes
Description	The biogenesis of mammalian autophagosomes remains to be fully defined. Here we used cellular and in vitro membrane fusion analyses to show that autophagosomes are formed from a hitherto unappreciated hybrid membrane compartment. The autophagic precursors emerge through fusion of FIP200 vesicles, derived from the cis-Golgi, with endosomally-derived ATG16L1 membranes to generate a hybrid pre-autophagosomal structure, HyPAS. HyPAS biogenesis depends on a previously unrecognized machinery centered upon STX17 interactors, the calcium pump SERCA2, extended synaptotagmin E-SYT2, and SIGMAR1. This apparatus controls HyPAS biogenesis and defines the mammalian autophagosomal precursor membranes. HyPAS can be modulated by pharmacological agents whereas its formation is inhibited upon SARS-CoV-2 infection or by expression of SARS-CoV-2 nsp6. These findings reveal the origin of mammalian autophagosomal membranes that emerge via convergence of secretory and endosomal pathways, and that this process is targeted by microbial factors such as coronaviral membrane modulating proteins.
HostingRepository	MassIVE
AnnounceDate	2021-11-09
AnnouncementXML	Submission_2021-11-09_11:38:31.628.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brett Phinney
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-16 15:40:12	ID requested
⏵ 1	2021-11-09 11:38:31	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: autophagosomes, HyPAS

submitter keyword: autophagosomes, HyPAS

Contact List

Vojo Deretic
contact affiliation	University of New Mexico Health Sciences Center
contact email	vderetic@salud.unm.edu
lab head
Brett Phinney
contact affiliation	UC Davis
contact email	brettsp@ucdavis.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087840/