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PXD027366

PXD027366 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMyxopyronin B inhibits growth of a Fidaxomicin-resistant Clostridioides difficile isolate and interferes with toxin synthesis
DescriptionFidaxomicin is considered the current gold standard antibiotic for treating Clostridioides difficile infections and kills bacterial cells by inhibition of the RNA polymerase through binding to its switch region. Although binding sites do not overlap, also Myxopyronin B inhibits the RNA polymerase by binding its switch region. The here presented data prove that there is no cross-resistance between Fidaxomicin and Myxopyronin B in a Fidaxomicin-resistant C. difficile strain. Moreover, comparative LC-MS/MS analyses of Fidaxomicin, Myxopyronin B and Rifaximin stress in C. difficile strain 630 revealed that Myxopyronin B is able to suppress early phase toxin synthesis in C. difficile to the same degree as Fidaxomicin. Conclusively, Myxopyronin B is proposed as lead structure for the design of novel antibiotics for the therapy of C. difficile infections.
HostingRepositoryPRIDE
AnnounceDate2021-12-21
AnnouncementXMLSubmission_2021-12-21_00:03:24.024.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaniela Zuehlke
SpeciesList scientific name: Clostridioides difficile 630; NCBI TaxID: 272563;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-16 07:41:05ID requested
12021-12-21 00:03:24announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: RNA polymerase inhibitors, Rifaximin, Fidaxomicin, Myxopyronin B, Clostridioides difficile, antibiotic therapy, antibiotic resistance, toxins
Contact List
Susanne Sievers
contact affiliationUniversity of Greifswald, Institute of Microbiology, Department of Microbial Physiology and Molecular Biology
contact emailsieverss@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliationUniversity of Greifswald, Institute of Microbiology
contact emailzuehlke@uni-greifswald.de
dataset submitter
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Dataset FTP location
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