PXD027366 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Myxopyronin B inhibits growth of a Fidaxomicin-resistant Clostridioides difficile isolate and interferes with toxin synthesis |
Description | Fidaxomicin is considered the current gold standard antibiotic for treating Clostridioides difficile infections and kills bacterial cells by inhibition of the RNA polymerase through binding to its switch region. Although binding sites do not overlap, also Myxopyronin B inhibits the RNA polymerase by binding its switch region. The here presented data prove that there is no cross-resistance between Fidaxomicin and Myxopyronin B in a Fidaxomicin-resistant C. difficile strain. Moreover, comparative LC-MS/MS analyses of Fidaxomicin, Myxopyronin B and Rifaximin stress in C. difficile strain 630 revealed that Myxopyronin B is able to suppress early phase toxin synthesis in C. difficile to the same degree as Fidaxomicin. Conclusively, Myxopyronin B is proposed as lead structure for the design of novel antibiotics for the therapy of C. difficile infections. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-21 |
AnnouncementXML | Submission_2021-12-21_00:03:24.024.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniela Zuehlke |
SpeciesList | scientific name: Clostridioides difficile 630; NCBI TaxID: 272563; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-16 07:41:05 | ID requested | |
⏵ 1 | 2021-12-21 00:03:24 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: RNA polymerase inhibitors, Rifaximin, Fidaxomicin, Myxopyronin B, Clostridioides difficile, antibiotic therapy, antibiotic resistance, toxins |
Contact List
Susanne Sievers |
contact affiliation | University of Greifswald, Institute of Microbiology, Department of Microbial Physiology and Molecular Biology |
contact email | sieverss@uni-greifswald.de |
lab head | |
Daniela Zuehlke |
contact affiliation | University of Greifswald, Institute of Microbiology |
contact email | zuehlke@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD027366 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027366
- Label: PRIDE project
- Name: Myxopyronin B inhibits growth of a Fidaxomicin-resistant Clostridioides difficile isolate and interferes with toxin synthesis