PXD027318 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Senescence Biomarker Discovery by LC-MS-based Proteomics |
Description | Senescence is a permanent cell cycle arrest that occurs in response to cellular stress. Because senescent cells promote age-related disease, there has been considerable interest in defining the proteomic alterations in senescent cells. Because senescence differs greatly depending on cell type and senescence inducer, continued progress in the characterization of senescent cells is needed. Here, we analyzed primary human mammary epithelial cells (HMECs), a model system for aging, using mass spectrometry-based proteomics. By integrating data from replicative senescence, immortalization by telomerase reactivation, and drug-induced senescence, we identified a robust proteomic signature of HMEC senescence consisting of 77 upregulated and 36 downregulated proteins. This approach identified known biomarkers, such as downregulation of the nuclear lamina protein lamin-B1 (LMNB1), and novel upregulated proteins including the β-galactoside-binding protein galectin-7 (LGALS7). Gene ontology enrichment analysis demonstrated that senescent HMECs upregulated lysosomal proteins and downregulated RNA metabolic processes. We additionally integrated our proteomic signature of senescence with transcriptomic data from senescent HMECs to demonstrate that our proteomic signature can discriminate proliferating and senescent HMECs even at the transcriptional level. Taken together, our results demonstrate the power of proteomics to identify cell type-specific signatures of senescence and advance the understanding of senescence in primary HMECs. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-28 |
AnnouncementXML | Submission_2021-09-28_09:17:11.451.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alireza Delfarah |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-14 22:12:53 | ID requested | |
⏵ 1 | 2021-09-28 09:17:11 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: senescence, aging, proteomics, mammary epithelial cells |
Contact List
Nicholas Alexander Graham |
contact affiliation | Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, USA |
contact email | nagraham@usc.edu |
lab head | |
Alireza Delfarah |
contact affiliation | University of Southern California |
contact email | delfarah@usc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027318
- Label: PRIDE project
- Name: Senescence Biomarker Discovery by LC-MS-based Proteomics