PXD027265 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A universal peptide matrix interactomics approach to disclose motif dependent protein binding |
Description | Protein-protein interactions (PPIs) mediated by intrinsically disordered regions (IDRs) are often based on short linear motifs (SLiM). SLiMs are implicated in signal transduction and gene regulation, yet remain technically laborious and notoriously challenging to study. Here, we present an optimized method for a PRotein Interaction Screen on a peptide MAtrix (PRISMA) in combination with quantitative mass spectrometry. The protocol was benchmarked with previously described SLiM based PPIs using peptides derived from EGFR, SOS1, GLUT1 and CEBPB and extended to map binding partners of kinase activation loops. The detailed protocol provides practical considerations for setting up a PRISMA screen and subsequently implementing PRISMA on a liquid handling robotic platform as a cost effective high-throughput method. Optimized PRISMA can be universally applied to systematically study SLiM based interactions and associated post translational modifications (PTMs) or mutations to advance our understanding of the largely uncharacterized interactomes of intrinsically disordered protein regions. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-11 |
AnnouncementXML | Submission_2021-08-10_23:46:39.364.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Evelyn Ramberger |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-13 05:15:50 | ID requested | |
⏵ 1 | 2021-08-10 23:46:39 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: peptide interactomics, PTM, PRISMA |
Contact List
Philipp Mertins |
contact affiliation | Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany |
contact email | Philipp.Mertins@mdc-berlin.de |
lab head | |
Evelyn Ramberger |
contact affiliation | Max Delbrück Center for Molecular Medicine, Berlin |
contact email | evelyn.ramberger@mdc-berlin.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD027265
- Label: PRIDE project
- Name: A universal peptide matrix interactomics approach to disclose motif dependent protein binding