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PXD027265

PXD027265 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA universal peptide matrix interactomics approach to disclose motif dependent protein binding
DescriptionProtein-protein interactions (PPIs) mediated by intrinsically disordered regions (IDRs) are often based on short linear motifs (SLiM). SLiMs are implicated in signal transduction and gene regulation, yet remain technically laborious and notoriously challenging to study. Here, we present an optimized method for a PRotein Interaction Screen on a peptide MAtrix (PRISMA) in combination with quantitative mass spectrometry. The protocol was benchmarked with previously described SLiM based PPIs using peptides derived from EGFR, SOS1, GLUT1 and CEBPB and extended to map binding partners of kinase activation loops. The detailed protocol provides practical considerations for setting up a PRISMA screen and subsequently implementing PRISMA on a liquid handling robotic platform as a cost effective high-throughput method. Optimized PRISMA can be universally applied to systematically study SLiM based interactions and associated post translational modifications (PTMs) or mutations to advance our understanding of the largely uncharacterized interactomes of intrinsically disordered protein regions.
HostingRepositoryPRIDE
AnnounceDate2021-08-11
AnnouncementXMLSubmission_2021-08-10_23:46:39.364.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEvelyn Ramberger
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-13 05:15:50ID requested
12021-08-10 23:46:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: peptide interactomics, PTM, PRISMA
Contact List
Philipp Mertins
contact affiliationMax Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany
contact emailPhilipp.Mertins@mdc-berlin.de
lab head
Evelyn Ramberger
contact affiliationMax Delbrück Center for Molecular Medicine, Berlin
contact emailevelyn.ramberger@mdc-berlin.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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