PXD027229

PXD027229 is an original dataset announced via ProteomeXchange.

Title	Characterisation of the free and membrane-associated fractions of the thylakoid lumen proteome in Arabidopsis thaliana
Description	In plants chloroplasts, the lumen of the thylakoid membrane houses proteins that are vital for photosynthetic electron transport, including water-splitting at photosystem (PS) II and shuttling of electrons from cytochrome b6f to PSI. Other lumen proteins maintain photosynthetic activity through biogenesis and turnover of PSII complexes. Although all lumen proteins are soluble, these known details have highlighted interactions of some lumen proteins with thylakoid membranes or thylakoid-intrinsic proteins. Meanwhile, the functional details of most lumen proteins, as well as their distribution between the soluble and membrane-associated lumen fractions, remain unknown. The current study isolated the soluble free lumen (FL) and membrane-associated lumen (MAL) fractions from Arabidopsis thaliana, and used gel- and mass spectrometry-based proteomics methods to analyse the contents of each proteome. These results identified lumenal proteins, and clearly distinguished the difference between the FL and MAL proteomes. The most abundant proteins in the FL fraction were involved in PSII assembly and repair, while the MAL proteome was enriched in proteins that support of the oxygen-evolving complex (OEC). Novel proteins, including a new PsbP domain-containing isoform, as well as several novel post-translational modifications and N-termini, are reported, and bi-dimensional separation of the lumen proteome identified several potential multi-protein oligomers in the thylakoid lumen.
HostingRepository	PRIDE
AnnounceDate	2021-07-30
AnnouncementXML	Submission_2021-07-30_05:02:15.922.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027229
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Trotta
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-12 01:42:02	ID requested
⏵ 1	2021-07-30 05:02:16	announced

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana, photosynthesis, chloroplast, thylakoid lumen, post-translational modification

Eva-Mari Aro
contact affiliation	Molecular Plant Biology, University of Turku, Finland
contact email	evaaro@utu.fi
lab head
Andrea Trotta
contact affiliation	Institute of Biosciences and Bioresources, National Research Council of Italy, Sesto Fiorentino (FI)
contact email	andrea.trotta@utu.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027229
     1. Label: PRIDE project
     2. Name: Characterisation of the free and membrane-associated fractions of the thylakoid lumen proteome in Arabidopsis thaliana