PXD027156

PXD027156 is an original dataset announced via ProteomeXchange.

Title	Cysteamine inhibits glycine utilisation and disrupts virulence in Pseudomonas aeruginosa
Description	Pseudomonas aeruginosa is a major opportunistic human pathogen which employs a myriad of virulence factors. In people with cystic fibrosis (CF) P. aeruginosa frequently colonises the lungs and becomes a chronic infection that evolves to become less virulent over time, but often adapts to favour persistence in the host with alginate-producing mucoid, slow-growing, and antibiotic resistant phenotypes emerging. Cysteamine is an endogenous aminothiol which has been shown to prevent biofilm formation, reduce phenazine production, and potentiate antibiotic activity against P. aeruginosa, and has been investigated in clinical trials as an adjunct therapy for pulmonary exacerbations of CF. Here we demonstrate (for the first time in a prokaryote) that cysteamine prevents glycine utilisation by P. aeruginosa in common with reported activity blocking the glycine cleavage system in human cells. Despite the inhibition of glycine metabolism, cysteamine inhibits hydrogen cyanide (HCN) production by P. aeruginosa, implicating interference in the regulation of virulence. Cysteamine impaired chemotaxis and reductions in pyocyanin and exopolysaccharide production in cysteamine-treated P. aeruginosa and reduced toxicity of secreted factors in a Galleria mellonella model. Thus, cysteamine has additional potent anti-virulence properties targeting P. aeruginosa, further supporting its therapeutic potential in CF and other infections.
HostingRepository	PRIDE
AnnounceDate	2021-08-19
AnnouncementXML	Submission_2021-08-19_08:02:05.161.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kevin Mclean
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-07 04:07:29	ID requested
⏵ 1	2021-08-19 08:02:05	announced

Dataset with its publication pending

submitter keyword: Pseudomonas aeruginosa , Human

Kevin Mclean
contact affiliation	Moredun Proteomics Facility Moredun Research Institute
contact email	kevin.mclean@moredun.ac.uk
lab head
Kevin Mclean
contact affiliation	Moredun Resaerch INstitute
contact email	kevin.mclean@moredun.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027156
     1. Label: PRIDE project
     2. Name: Cysteamine inhibits glycine utilisation and disrupts virulence in Pseudomonas aeruginosa