PXD027105

PXD027105 is an original dataset announced via ProteomeXchange.

Title	Evidence of immune modulators in the secretome of the equine tapeworm Anoplocephala perfoliata
Description	Anoplocephala perfoliata is a neglected gastro-intestinal tapeworm, commonly infecting horses worldwide. Molecular investigation of A. perfoliata is hampered by a lack of tools to better un-derstand the host-parasite interface. This interface is likely influenced by parasite derived immune modulators released in the secretome as free proteins or components of extracellular vesicles (EVs). Therefore, adult RNA was sequenced and de novo assembled to generate the first A. perfoliata transcriptome. In addition, excretory secretory products (ESP) from adult A. perfoliata were col-lected and EVs isolated using size exclusion chromatography, prior to proteomic analysis of the EVs, the EV surface and EV depleted ESP. Transcriptome analysis revealed 454 sequences ho-mologous to known helminth immune modulators including 2 novel Sigma class GSTs, 5 α-HSP90s and 3 α-enolases with isoforms of all three observed within the proteomic analysis of the secretome. Furthermore, secretome proteomics identified common helminth proteins across each sample with known EV markers, such as annexins and tetraspanins, observed in EV fractions. Importantly, 49 of the 454 putative immune modulators were identified across the secretome proteomics contained within and on the surface of EVs in addition to those identified in free ESP. This work provides the molecular tools for A. perfoliata to reveal key players in the host-parasite interaction within the horse host.
HostingRepository	PRIDE
AnnounceDate	2021-07-26
AnnouncementXML	Submission_2021-07-26_10:07:12.679.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027105
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Russ Morphew
SpeciesList	scientific name: Anoplocephala perfoliata; NCBI TaxID: 218192;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-05 08:40:14	ID requested
⏵ 1	2021-07-26 10:07:13	announced

Dataset with its publication pending

submitter keyword: Anoplocephala perfoliata

Secretome

Extracellular Vesicles

EV Surface

EV depleted ESP

LCMSMS

Russell M Morphew
contact affiliation	Institute of Biological, Environmental & Rural Sciences (IBERS,)Aberystwyth University, Wales, UK.
contact email	rom@aber.ac.uk
lab head
Russ Morphew
contact affiliation	Aberystwyth University
contact email	rom@aber.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027105
     1. Label: PRIDE project
     2. Name: Evidence of immune modulators in the secretome of the equine tapeworm Anoplocephala perfoliata