PXD027055 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Early and late processes driving NET formation, and the autocrine/paracrine role of endogenous RAGE ligands |
Description | Neutrophil extracellular trap (NET) formation has emerged as an important response against various pathogens; it also plays a role in chronic inflammation, autoimmunity, and cancer. Despite a growing understanding of the mechanisms underlying NET formation, much remains to be elucidated. We previously showed that in human neutrophils activated with different classes of physiological stimuli, NET formation features both early and late events that are controlled by discrete signaling pathways. However, the nature of these events has remained elusive. We now report that PAD4 inhibition only affects the early phase of NETosis, as do distinct signaling intermediates (TAK1, MEK, p38 MAPK). Accordingly, the inducible citrullination of residue R2 on histone H3 is an early neutrophil response that is regulated by these kinases; other arginine residues on histones H3 and H4 do not seem to be citrullinated. Conversely, chromatin decondensation is a belated event in NET formation, that is impaired by the inhibition of most signaling pathways known to control the phenomenon. We additionally show that neutrophils can condition themselves to be poised for rapid NET formation, and that this represents another late process. Similarly, activated neutrophils release a number of endogenous proteic factors that promote and largely mediate NET generation, and this is yet another late process. Our data shed new light on the cellular processes underlying NET formation, and unveil some unsuspected facets of the phenomenon. In view of the involvement of NETs in both homeostasis and several pathologies, our findings are of broad relevance. |
HostingRepository | PRIDE |
AnnounceDate | 2021-10-12 |
AnnouncementXML | Submission_2021-10-12_05:34:53.151.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Francois-Michel Boisvert |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-01 23:31:49 | ID requested | |
⏵ 1 | 2021-10-12 05:34:53 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Neutrophil, NET formation, activation, inflammation, autoimmunity |
Contact List
Patrick McDonald |
contact affiliation | Department of Medecine, Service of Pneumology, Université de Sherbrooke |
contact email | Patrick.McDonald@usherbrooke.ca |
lab head | |
Francois-Michel Boisvert |
contact affiliation | Université de Sherbrooke |
contact email | fm.boisvert@usherbrooke.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027055
- Label: PRIDE project
- Name: Early and late processes driving NET formation, and the autocrine/paracrine role of endogenous RAGE ligands