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PXD027026

PXD027026 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTwo related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through two different acetyltransferase-independent mechanisms
DescriptionSAGA and ATAC are two related transcriptional coactivator complexes, sharing the same histone acetyltransferase (HAT) subunit. The HAT activities of SAGA and ATAC are required for metazoan development but the precise role of the two complexes in RNA polymerase II transcription in mammals is less understood. To determine whether SAGA and ATAC have redundant or specific functions dependent on their HAT activities, we compared the effects of HAT inactivation in each complex with that of inactivation of either SAGA or ATAC core subunits in mouse embryonic stem cells (ESCs). We show that core subunits of SAGA or ATAC subunits are required for complex assembly, mouse ESC growth and self-renewal. Additionally, ATAC, but not SAGA subunits are required for ESC viability by regulating the transcription of translation-related genes. Surprisingly, depletion of specific or shared HAT module subunits caused a global decrease in histone H3K9 acetylation, but did not result in significant phenotypic or transcriptional defects. Thus, our results indicate that SAGA and ATAC are differentially required for viability and self-renewal of mouse ESCs by regulating transcription through different pathways, in a HAT-independent manner.
HostingRepositoryPRIDE
AnnounceDate2021-08-11
AnnouncementXMLSubmission_2021-08-11_05:31:35.322.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuc Negroni
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-30 22:39:59ID requested
12021-08-11 05:31:35announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mouse embryonic stem cells, coactivator complexes, SAGA, ATAC, Pol II transcription, 4sU labelling, newly synthesized RNA, histone acetyltransferase, HAT-independent function
Contact List
Didier Devys
contact affiliationInstitut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch CEDEX, France
contact emaildevys@igbmc.fr
lab head
Luc Negroni
contact affiliationCNRS
contact emailluc.negroni@igbmc.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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