PXD027019
PXD027019 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Vitamin C differentially impacts the serum proteome profile in female and male mice |
| Description | Suboptimal blood vitamin C (ascorbate) level increases the risk of several chronic diseases. However, the detection of hypovitaminosis C is not a simple task as ascorbate is unstable in blood samples. In this study, we examined the serum proteome of mice lacking the enzyme gulonolactone oxidase (Gulo) required for the biosynthesis of ascorbate. Gulo-/- mice were supplemented with different concentrations of ascorbate in drinking water and serum was collected to identify proteins correlating with serum ascorbate levels using an LC-MS/MS quantitative proteomic approach. Parallel reaction monitoring was performed to validate the correlations. We uncovered that the serum proteome profiles differ significantly between male and female mice. Also, unlike Gulo-/- males, a four-week treatment of ascorbate did not entirely re-establish the serum proteome profile of ascorbate-deficient Gulo-/- females to the optimal profile exhibited by Gulo-/- females that never experienced an ascorbate deficiency. Finally, serum proteins involved in retinoid metabolism, cholesterol, and lipid transport were similarly affected by ascorbate levels in males and females. In contrast, proteins regulating serum peptidases and protein of the acute phase response were different between males and females. These proteins are potential biomarkers correlating with blood ascorbate levels and require further study in standard clinical settings. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-09-30 |
| AnnouncementXML | Submission_2024-09-30_07:45:06.767.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Michel Lebel |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-06-30 09:02:28 | ID requested | |
| ⏵ 1 | 2024-09-30 07:45:07 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: vitamin C, gulonolactone oxidase, mass spectrometry, proteomics, serum, sex related differences, mouse |
Contact List
| Michel Lebel | |
|---|---|
| contact affiliation | Centre Hospitalier Universite Laval |
| contact email | michel.lebel@crchudequebec.ulaval.ca |
| lab head | |
| Michel Lebel | |
| contact affiliation | CHudeQuebe |
| contact email | michel.lebel@crchudequebec.ulaval.ca |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v03/MSV000087709/ |
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