PXD026998 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pro-inflammatory Beta Cell Small Extracellular Vesicles Induce Beta Cell Failure Through Activation of the CXCL10/CXCR3 Axis in Diabetes |
Description | Coordinated communication among pancreatic islet cells is necessary for the maintenance of glucose homeostasis. In diabetes, chronic exposure to pro-inflammatory cytokines has been shown to perturb β-cell communication and function. Compelling evidence has implicated extracellular vesicles (EVs) in modulating physiological and pathological responses to β-cell stress. We report that pro-inflammatory β-cell small EVs (cytoEV) induce β-cell dysfunction, promote a pro-inflammatory islet transcriptome, and enhance recruitment of CD8+ T-cells and macrophages. Proteomic analysis of cytoEV revealed an enrichment of the chemokine, CXCL10, with surface topological analysis depicting CXCL10 as membrane-bound on cytoEV to facilitate direct binding to CXCR3 receptors on the surface of β-cells. CXCR3 receptor inhibition reduced CXCL10-cytoEV binding and attenuated β-cell dysfunction, inflammatory gene expression, and leukocyte recruitment to islets. Collectively, this work implicates the significant role of pro-inflammatory β-cell derived small EVs in modulating β-cell function, global gene expression, and antigen presentation through activation of the CXCL10/CXCR3 axis. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-25 |
AnnouncementXML | Submission_2021-08-25_05:47:12.488.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Naureen Javeed |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-29 21:15:52 | ID requested | |
⏵ 1 | 2021-08-25 05:47:12 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Extracellular vesicles, β-cell, inflammation, diabetes, intercellular communication, CXCL10 |
Contact List
Naureen Javeed |
contact affiliation | Mayo Clinic Department of Physiology and Biomedical Engineering Rochester, MN 55901 |
contact email | javeed.naureen@mayo.edu |
lab head | |
Naureen Javeed |
contact affiliation | Mayo Clinic |
contact email | javeed.naureen@mayo.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD026998 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026998
- Label: PRIDE project
- Name: Pro-inflammatory Beta Cell Small Extracellular Vesicles Induce Beta Cell Failure Through Activation of the CXCL10/CXCR3 Axis in Diabetes