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PXD026998

PXD026998 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePro-inflammatory Beta Cell Small Extracellular Vesicles Induce Beta Cell Failure Through Activation of the CXCL10/CXCR3 Axis in Diabetes
DescriptionCoordinated communication among pancreatic islet cells is necessary for the maintenance of glucose homeostasis. In diabetes, chronic exposure to pro-inflammatory cytokines has been shown to perturb β-cell communication and function. Compelling evidence has implicated extracellular vesicles (EVs) in modulating physiological and pathological responses to β-cell stress. We report that pro-inflammatory β-cell small EVs (cytoEV) induce β-cell dysfunction, promote a pro-inflammatory islet transcriptome, and enhance recruitment of CD8+ T-cells and macrophages. Proteomic analysis of cytoEV revealed an enrichment of the chemokine, CXCL10, with surface topological analysis depicting CXCL10 as membrane-bound on cytoEV to facilitate direct binding to CXCR3 receptors on the surface of β-cells. CXCR3 receptor inhibition reduced CXCL10-cytoEV binding and attenuated β-cell dysfunction, inflammatory gene expression, and leukocyte recruitment to islets. Collectively, this work implicates the significant role of pro-inflammatory β-cell derived small EVs in modulating β-cell function, global gene expression, and antigen presentation through activation of the CXCL10/CXCR3 axis.
HostingRepositoryPRIDE
AnnounceDate2021-08-25
AnnouncementXMLSubmission_2021-08-25_05:47:12.488.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNaureen Javeed
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-29 21:15:52ID requested
12021-08-25 05:47:12announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Extracellular vesicles, β-cell, inflammation, diabetes, intercellular communication, CXCL10
Contact List
Naureen Javeed
contact affiliationMayo Clinic Department of Physiology and Biomedical Engineering Rochester, MN 55901
contact emailjaveed.naureen@mayo.edu
lab head
Naureen Javeed
contact affiliationMayo Clinic
contact emailjaveed.naureen@mayo.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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