PXD026930 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Impact of Alanyl-tRNA Synthetase Editing Deficiency in Yeast |
Description | Aminoacyl-tRNA synthetases (aaRSs) are essential enzymes that provide the ribosome with aminoacyl-tRNA substrates for protein synthesis. Mutations in aaRSs lead to various neurological disorders in humans. Many aaRSs utilize editing to prevent error propagation during translation. Editing defects in alanyl-tRNA synthetase (AlaRS) cause neurodegeneration and cardioproteinopathy in mice and is associated with microcephaly in human patients. The cellular impact of AlaRS editing deficiency in eukaryotes remains unclear. Here we use yeast as a model organism to systematically investigate the physiological role of AlaRS editing. Our RNA sequencing and quantitative proteomics analyses reveal that AlaRS editing defects surprisingly activate the general amino acid control pathway and attenuate the heatshock response. We have confirmed these results with reporter and growth assays. In addition, AlaRS editing defects downregulate carbon metabolism and attenuate protein synthesis. Supplying yeast cells with extra carbon source partially rescues the heat sensitivity caused by AlaRS editing deficiency. These findings are in stark contrast with the cellular effects caused by editing deficiency in other aaRSs. Our study therefore highlights the idiosyncratic role of AlaRS editing compared with other aaRSs and provides a model for the physiological impact caused by the lack of AlaRS editing. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-26 |
AnnouncementXML | Submission_2021-08-26_05:20:23.035.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jiqiang Ling |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-25 03:54:51 | ID requested | |
⏵ 1 | 2021-08-26 05:20:23 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: yeast AlaRS variants, label-free quantitative proteomics |
Contact List
Jiqiang Ling |
contact affiliation | Department of Cell Biology and Molecular Genetics University of Maryland |
contact email | jling12@umd.edu |
lab head | |
Jiqiang Ling |
contact affiliation | University of Maryland |
contact email | jling12@umd.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026930
- Label: PRIDE project
- Name: Impact of Alanyl-tRNA Synthetase Editing Deficiency in Yeast