PXD026843 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The E3 ubiquitin ligase RNF115 regulates phagosome maturation and host response to infection |
Description | Phagocytosis is an essential process for the microbicidal function of professional phagocytes, in which phagosome maturation plays a critical role. Macrophage activation by interferons (IFN) increases their microbicidal activity, but delays phagosomal maturation. Here, we investigated the role of ubiquitylation in phagosome functions. We show that phagosomal proteins are ubiquitylated and that phagosomes contain diverse ubiquitin chain types, including atypical ubiquitin chains. IFN-γ activation of macrophages substantially enhanced phagosomal ubiquitylation of both innate immune response proteins and vesicle trafficking proteins. We identified the E3 ubiquitin ligase RNF115, which is enriched on phagosomes of IFN-γ activated macrophages, as an important regulator of phagosomal maturation. Loss of RNF115 protein or ubiquitin ligase activity facilitated enhanced phagosomal maturation, and increased cytokine responses to Staphylococcus aureus. Our data suggests that both innate immune signalling and phagolysosomal trafficking are controlled through ubiquitylation. In conclusion, we identify RNF115 and ubiquitylation as important regulators of innate immune signalling from the phagosome during bacterial infections. |
HostingRepository | PRIDE |
AnnounceDate | 2022-07-20 |
AnnouncementXML | Submission_2022-07-20_05:18:17.813.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matthias Trost |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | ubiquitinylated lysine |
Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-21 06:03:20 | ID requested | |
⏵ 1 | 2022-07-20 05:18:18 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Phagosome, Macrophage, Ubiquitin, E3-ligase, RNF115 |
Contact List
Matthias Trost |
contact affiliation | Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom |
contact email | matthias.trost@ncl.ac.uk |
lab head | |
Matthias Trost |
contact affiliation | Newcastle University |
contact email | matthias.trost@ncl.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026843
- Label: PRIDE project
- Name: The E3 ubiquitin ligase RNF115 regulates phagosome maturation and host response to infection