PXD026776

PXD026776 is an original dataset announced via ProteomeXchange.

Title	Profiling of glioma C6 and astrocytes rat cell lines before and after co-cultivation.
Description	We have grown C6 glioma cells and rat astrocytes, as well as astrocyte cells co-cultured together with C6 glioma. We performed proteome-wide LC-MS analysis of this experimental groups. The data including LC-MS/MS raw files and exported MaxQuant report. For our co-cultivated in vitro model we used astrocytes and C6 glioma cells. Astrocytes cell lines isolated from rat brain tissue. We analyzed astrocytes in two conditions: beforeand after co-cultivation. Proteins were assessed in an untargeted label-free bottom-up proteomic experiment using IDA approach (i.e. InformationDependent Acquisition) on AB Sciex TripleTOF 6600 Q-TOF mass-spectrometer coupled with LFQ (label-free quantification) approach by MaxQuant software. Dataset covers 165 samples (11 biological rand 5 technical replicates)
HostingRepository	PRIDE
AnnounceDate	2021-10-05
AnnouncementXML	Submission_2021-10-05_03:46:34.568.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olga Bukato
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-18 01:36:31	ID requested
⏵ 1	2021-10-05 03:46:34	announced

Dataset with its publication pending

submitter keyword: glioma C6, co-culture, LC-MS/MS, proteome

Artemiy S. Silantyev
contact affiliation	Federal State Institution V. P. Serbsky Federal Medical Research Center of Psychiatry and Narcology National Scientific Research Centre on Addictions of the Ministry of Healthcare of the Russian Federation, 119002 Moscow, Russian Federation
contact email	artsilan@gmail.com
lab head
Olga Bukato
contact affiliation	Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
contact email	olya.bukato@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD026776

PRIDE project URI

• PRIDE
  1. PXD026776
     1. Label: PRIDE project
     2. Name: Profiling of glioma C6 and astrocytes rat cell lines before and after co-cultivation.