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PXD026763

PXD026763 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman MDA-MB-231 breast cancer cells overexpressing miR-183-5p and its isomiRs
DescriptionOverexpression of miR-183-5p|+2, but not of the other two isomiRs |0 and |+1, was observed to reduce cell cycle and cell proliferation in different triple-negative breast cancer cell lines. Therefore, we hypothesized that the |+2 isoform has targets distinct from the other two isoforms. To test this hypothesis, we overexpressed separately the three different isoforms or negative controls (siAllstar or mimic-Cltr) and performed Mass Spectrometry to identify differentially regulated proteins. Interestingly, a gene set enrichment analysis of the changes in protein expression revealed significant downregulation of transcriptional targets of E2F specifically in cells transfected with the |+2 isoform prompting us to validate the predicted isomiR specific target E2F1. Subsequently, we could show that direct targeting of E2F1 by miR-183-5p|+2 is responsible for the impact of the isomiR on cell cycle and proliferation.
HostingRepositoryPRIDE
AnnounceDate2022-05-27
AnnouncementXMLSubmission_2022-05-26_16:05:54.425.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCindy Körner
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-17 21:36:13ID requested
12022-05-26 16:05:54announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Human, breast cancer, MDA-MB-231, miRNA, miR-183-5p
Contact List
Stefan Wiemann
contact affiliationMolecular Genome Analysis, German Cancer Research Centre, Heidelberg, Germany
contact emails.wiemann@dkfz.de
lab head
Cindy Körner
contact affiliationGerman Cancer Research Center, Heidelberg Molecular Genome Analysis (B050)
contact emailc.koerner@dkfz-heidelberg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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