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PXD026725

PXD026725 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRNA-bound PGC-1α controls gene expression in liquid-like nuclear condensates
DescriptionThe peroxisome-proliferator-activated receptor-γ coactivator-1α (PGC-1α) integrates environmental cues by controlling complex transcriptional networks in various metabolically active tissues. However, it is unclear how a transcriptional coregulator coordinates dynamic biological programs in response to multifaceted stimuli such as endurance training or fasting. Here, we discovered a central function of the poorly understood C-terminal domain (CTD) of PGC-1α to bind RNAs and assemble multi-protein complexes. Surprisingly, in addition to controlling the coupling of transcription and processing of target genes, RNA binding is indispensable for the recruitment of PGC-1α to chromatin into liquid-like nuclear condensates, which compartmentalize and regulate active transcription. These results demonstrate a hitherto unsuspected molecular mechanism by which complexity in the regulation of large transcriptional networks by PGC-1α is achieved. These findings are not only essential for the basic understanding of transcriptional coregulator-driven control of biological programs, but will also help to devise new strategies to modulate these processes in pathological contexts in which PGC-1α function is dysregulated, such as type 2 diabetes, cardiovascular diseases or skeletal muscle wasting.
HostingRepositoryPRIDE
AnnounceDate2021-08-19
AnnouncementXMLSubmission_2021-08-19_05:30:17.036.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD026725
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDanilo Ritz
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Orbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-15 14:37:07ID requested
12021-08-19 05:30:17announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Muscle cells, APMS, LCMS/MS
Contact List
Alexander Schmidt
contact affiliationDr. Alexander Schmidt Proteomics Core Facility Biozentrum, University of Basel Klingelbergstrasse 50/70 CH-4056 Basel / Switzerland
contact emailalex.schmidt@unibas.ch
lab head
Danilo Ritz
contact affiliationProteomics Core Facility Biozentrum, University of Basel Klingelbergstrasse 50/70 CH-4056 Basel / Switzerland
contact emaildanilo.ritz@unibas.ch
dataset submitter
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Dataset FTP location
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