PXD026704 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Coating and corruption of human neutrophils by bacterial outer membrane vesicles |
Description | Porphyromonas gingivalis is a keystone oral pathogen that successfully manipulates the human innate immune defenses, resulting in a chronic pro-inflammatory state of periodontal tissues and beyond. Here we demonstrate that secreted outer membrane vesicles (OMVs) are deployed by P. gingivalis to selectively coat and activate human neutrophils, thereby provoking degranulation without neutrophil killing. Secreted granule components with antibacterial activity, especially LL-37 and MPO, are subsequently degraded by potent OMV-bound proteases known as gingipains, thereby ensuring bacterial survival. In contrast to neutrophils, the P. gingivalis OMVs are efficiently internalized by macrophages and epithelial cells. Importantly, we show that neutrophil coating is a conserved feature displayed by OMVs of at least one other oral pathogen, namely Aggregatibacter actinomycetemcomitans. Altogether, we conclude that P. gingivalis deploys its OMVs for a neutrophil-deceptive strategy to escape phagocytosis and to create a favorable inflammatory niche. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-16 |
AnnouncementXML | Submission_2022-08-16_13:14:47.466.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Claudia Hirschfeld |
SpeciesList | scientific name: Porphyromonas gingivalis W83; NCBI TaxID: 242619; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue; L-citrulline |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-14 23:50:41 | ID requested | |
⏵ 1 | 2022-08-16 13:14:47 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: OMVs, Porphyromonas gingivalis, neutrophils, myeloperoxidase, citrullination, gingipains, LL-37 |
Contact List
Prof. Dr. Dörte Becher |
contact affiliation | University of Greifswald, Center for Functional Genomics of Microbes, Institute for Microbiology, Department of Microbial Proteomics, Greifswald, Germany |
contact email | dbecher@uni-greifswald.de |
lab head | |
Claudia Hirschfeld |
contact affiliation | University of Greifswald |
contact email | claudia.hirschfeld@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD026704 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026704
- Label: PRIDE project
- Name: Coating and corruption of human neutrophils by bacterial outer membrane vesicles