PXD026633 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation |
Description | This study tracks the proteome during recovery from 10 kGy acute ionizing radiation (IR) in Deinococcus radiodurans R1 (WT). After 1 hour of recovery post-IR exposure, we observed 37 proteins significantly differentially expressed, including several within the Radiation and Dessication Response (RDR) pathway. Additionally, we also explored the regulatory network of a sRNA named PprS (previously Dsr2) in Deinococcus radiodurans by comparing the proteome of a sRNA knockdown strain (PprSKD, which demonstrates a ~2-fold decrease in PprS expression) to WT D. radiodurans during unirradiated conditions at late-exponential phase. Comparison between these two strains demonstrated decreased levels of one of PprS's targets, PprM, in the PprSKD strain which validated the activation mechanism we propose for PprS on pprM. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-25 |
AnnouncementXML | Submission_2021-06-24_23:14:27.680.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jordan Villa |
SpeciesList | scientific name: Deinococcus radiodurans R1; NCBI TaxID: 243230; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-10 22:31:55 | ID requested | |
⏵ 1 | 2021-06-24 23:14:28 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: deinococcus, sRNA, ionizing radiation, IR, deinococcus radiodurans, shotgun proteomics, timecourse |
Contact List
Lydia M Contreras |
contact affiliation | McKetta Department of Chemical Engineering, The University of Texas at Austin, USA |
contact email | lcontrer@che.utexas.edu |
lab head | |
Jordan Villa |
contact affiliation | University of Texas at Austin |
contact email | jkvilla27@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD026633
- Label: PRIDE project
- Name: A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation