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PXD026633

PXD026633 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation
DescriptionThis study tracks the proteome during recovery from 10 kGy acute ionizing radiation (IR) in Deinococcus radiodurans R1 (WT). After 1 hour of recovery post-IR exposure, we observed 37 proteins significantly differentially expressed, including several within the Radiation and Dessication Response (RDR) pathway. Additionally, we also explored the regulatory network of a sRNA named PprS (previously Dsr2) in Deinococcus radiodurans by comparing the proteome of a sRNA knockdown strain (PprSKD, which demonstrates a ~2-fold decrease in PprS expression) to WT D. radiodurans during unirradiated conditions at late-exponential phase. Comparison between these two strains demonstrated decreased levels of one of PprS's targets, PprM, in the PprSKD strain which validated the activation mechanism we propose for PprS on pprM.
HostingRepositoryPRIDE
AnnounceDate2021-06-25
AnnouncementXMLSubmission_2021-06-24_23:14:27.680.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJordan Villa
SpeciesList scientific name: Deinococcus radiodurans R1; NCBI TaxID: 243230;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-10 22:31:55ID requested
12021-06-24 23:14:28announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: deinococcus, sRNA, ionizing radiation, IR, deinococcus radiodurans, shotgun proteomics, timecourse
Contact List
Lydia M Contreras
contact affiliationMcKetta Department of Chemical Engineering, The University of Texas at Austin, USA
contact emaillcontrer@che.utexas.edu
lab head
Jordan Villa
contact affiliationUniversity of Texas at Austin
contact emailjkvilla27@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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