PXD026622 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The RNA-binding protein RBP42 regulates cellular energy metabolism in mammalian-infective Trypanosoma brucei |
Description | RNA-binding proteins are key players in coordinated post-transcriptional regulation of functionally related genes, defined as RNA regulons. RNA regulons play particularly critical roles in parasitic trypanosomes, which exhibit unregulated co-transcription of long unrelated gene arrays. In this report, we present a systematic analysis of an essential RNA-binding protein, RBP42, in the mammalian-infective bloodstream form of African trypanosome, and show that RBP42 is a key regulator of parasite’s central carbon and energy metabolism. Using individual-nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) to identify genome-wide RBP42-RNA interactions, we show that RBP42 preferentially binds within the coding region of mRNAs encoding core metabolic enzymes. Global quantitative transcriptomic and proteomic analyses reveal that loss of RBP42 reduces the abundance of target mRNA-encoded proteins, but not target mRNA, suggesting a positive translational regulatory role of RBP42. Significant changes in central carbon metabolic intermediates, following loss of RBP42, further support its critical role in cellular energy metabolism. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-11 |
AnnouncementXML | Submission_2021-06-10_22:23:55.000.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD026622 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Tong Liu |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-10 09:03:55 | ID requested | |
⏵ 1 | 2021-06-10 22:23:55 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: RBP42, Trypanosoma brucei, LC-MS/MS, iTRAQ |
Contact List
Anish Das |
contact affiliation | Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers-New Jersey Medical School, Newark, NJ 07103 |
contact email | dasak@njms.rutgers.edu |
lab head | |
Tong Liu |
contact affiliation | Rutgers University |
contact email | linto@njms.rutgers.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026622
- Label: PRIDE project
- Name: The RNA-binding protein RBP42 regulates cellular energy metabolism in mammalian-infective Trypanosoma brucei