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PXD026619

PXD026619 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDynamic character displacement among a pair of bacterial phyllosphere commensals in situ
DescriptionDifferences between species promote stable coexistence in a resource-limited environment. These differences can result from interspecies competition leading to character shifts, a process referred to as character displacement. While character displacement is often interpreted as a consequence of genetically fixed trait differences between species, it can also be mediated by phenotypic plasticity in response to the presence of another species. Here, we test whether phenotypic plasticity leads to a shift in proteome allocation during co-occurrence of two bacterial species from the abundant, leaf-colonizing families Sphingomonadaceae and Rhizobiaceae in their natural habitat. Upon mono-colonizing of the phyllosphere, both species exhibit specific and shared protein functions indicating a niche overlap. During co-colonization, quantitative differences in the protein repertoire of both bacterial populations occur as a result of bacterial coexistence in planta. Specifically, the Sphingomonas strain produces enzymes for the metabolization of xylan, while the Rhizobium strain reprograms its metabolism to beta-oxidation of fatty acids fueled via the glyoxylate cycle and adapts its biotin acquisition. We demonstrate the conditional relevance of cross-species facilitation by mutagenesis leading to loss of fitness in competition in planta. Our results show that dynamic character displacement and niche facilitation mediated by phenotypic plasticity can contribute to species coexistence.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:56:51.146.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLucas Hemmerle
SpeciesList scientific name: Sphingomonas sp. Leaf257; NCBI TaxID: 1736309; scientific name: Rhizobium sp. Leaf68; NCBI TaxID: 1736231;
ModificationListcarbamoylated residue; iodoacetamide derivatized amino-terminal residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-10 07:54:25ID requested
12022-05-26 09:11:32announced
22023-11-14 08:56:51announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Sphingomonas,Phyllosphere, Dynamic character displacement, Rhizobium, Proteomics
Contact List
Julia A. Vorholt
contact affiliationDepartment of Biology, Institute of Microbiology, ETH Zürich, Switzerland
contact emailjvorholt@ethz.ch
lab head
Lucas Hemmerle
contact affiliationETH Zürich
contact emailhelucas@ethz.ch
dataset submitter
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Dataset FTP location
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