PXD026599 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A phosphoproteomic approach reveals that PKD3 controls PKA-mediated glucose and tyrosine metabolism |
Description | Members of the Protein Kinase D (PKD) family (PKD1, 2, and 3) integrate hormonal and nutritional inputs to regulate complex cellular metabolism. Despite the fact that a number of functions have been annotated to particular PKDs, their molecular targets are relatively poorly explored. PKD3 promotes insulin sensitivity and suppresses lipogenesis in the liver of animals fed a high-fat diet. However, its substrates are largely unknown. Here we applied proteomic approaches to determine PKD3 targets. We identified over three-hundred putative targets of PKD3. Further, biochemical analysis revealed that PKD3 regulates cAMP-dependent protein kinase A (PKA) activity, a master regulator of the hepatic response to glucagon and fasting. PKA regulates glucose, lipid, and amino acid metabolism in the liver, by targeting key enzymes in the respective processes. Among them the PKA targets phenylalanine hydroxylase (PAH) catalyzes the conversion of phenylalanine to tyrosine Consistently, we showed that PKD3 is activated by glucagon and promotes glucose and tyrosine levels in hepatocytes |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-17 |
AnnouncementXML | Submission_2021-06-16_22:52:05.966.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Angel Loza Valdes |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-09 06:15:19 | ID requested | |
⏵ 1 | 2021-06-16 22:52:06 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Primary mouse hepatocytes, PKD3, PKA |
Contact List
Grzegorz Sumara |
contact affiliation | nencki institute of experimental biology |
contact email | g.sumara@nencki.edu.pl |
lab head | |
Angel Loza Valdes |
contact affiliation | PhD Student |
contact email | a.loza-valdes@nencki.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026599
- Label: PRIDE project
- Name: A phosphoproteomic approach reveals that PKD3 controls PKA-mediated glucose and tyrosine metabolism