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PXD026599

PXD026599 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA phosphoproteomic approach reveals that PKD3 controls PKA-mediated glucose and tyrosine metabolism
DescriptionMembers of the Protein Kinase D (PKD) family (PKD1, 2, and 3) integrate hormonal and nutritional inputs to regulate complex cellular metabolism. Despite the fact that a number of functions have been annotated to particular PKDs, their molecular targets are relatively poorly explored. PKD3 promotes insulin sensitivity and suppresses lipogenesis in the liver of animals fed a high-fat diet. However, its substrates are largely unknown. Here we applied proteomic approaches to determine PKD3 targets. We identified over three-hundred putative targets of PKD3. Further, biochemical analysis revealed that PKD3 regulates cAMP-dependent protein kinase A (PKA) activity, a master regulator of the hepatic response to glucagon and fasting. PKA regulates glucose, lipid, and amino acid metabolism in the liver, by targeting key enzymes in the respective processes. Among them the PKA targets phenylalanine hydroxylase (PAH) catalyzes the conversion of phenylalanine to tyrosine Consistently, we showed that PKD3 is activated by glucagon and promotes glucose and tyrosine levels in hepatocytes
HostingRepositoryPRIDE
AnnounceDate2021-06-17
AnnouncementXMLSubmission_2021-06-16_22:52:05.966.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAngel Loza Valdes
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-09 06:15:19ID requested
12021-06-16 22:52:06announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Primary mouse hepatocytes, PKD3, PKA
Contact List
Grzegorz Sumara
contact affiliationnencki institute of experimental biology
contact emailg.sumara@nencki.edu.pl
lab head
Angel Loza Valdes
contact affiliationPhD Student
contact emaila.loza-valdes@nencki.edu.pl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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