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PXD026570

PXD026570 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExtensive Three-Dimensional Intratumor Proteomic Heterogeneity Revealed by Multiregion Sampling in High-Grade Serous Ovarian Tumor Specimens
DescriptionEnriched tumor epithelium, tumor-associated stroma, and whole tissue were collected by laser microdissection from thin sections across spatially separated levels of ten high-grade serous ovarian carcinomas (HGSOC) and analyzed by mass spectrometry. Unsupervised analyses of protein abundance data revealed independent clustering of enriched stroma and enriched tumor epithelium, with whole tumor tissue clustering driven by overall tumor “purity”. Comparing these data to previously defined prognostic HGSOC molecular subtypes revealed protein expression from tumor epithelium correlated with the differentiated subtype, whereas stromal proteins correlated with the mesenchymal subtype. Protein abundance in tumor epithelium and stroma exhibited decreased correlation in samples collected just hundreds of microns apart. These data reveal substantial tumor microenvironment protein heterogeneity that directly bears on prognostic signatures, biomarker discovery, and cancer pathophysiology, and underscore the need to enrich cellular subpopulations for expression profiling.
HostingRepositoryPRIDE
AnnounceDate2021-07-01
AnnouncementXMLSubmission_2021-06-30_23:16:33.934.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBrian Hood
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-08 09:04:39ID requested
12021-06-30 23:16:34announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: high grade serous ovarian cancer, proteomics, laser microdissection, tumor microenvironment, heterogeneity
Contact List
Thomas P. Conrads, PhD
contact affiliationDirector of Women’s Health Research Women’s Service Line Inova Health System Chief Scientific Officer Gynecologic Cancer Center of Excellence John P. Murtha Cancer Center Research Program Uniformed Services University of the Health Sciences
contact emailconrads@whirc.org
lab head
Brian Hood
contact affiliationWomen's Health Integrated Research Center
contact emailhoodb@whirc.org
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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