PXD026465 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Inositol signaling in the basidiomycete fungus Schizophyllum commune |
Description | Intracellular signaling is conserved in eukaryotes to allow for response to extracellular signals and to regulate development and cellular functions. In fungi, inositol phosphate signaling has been shown to be involved in growth, sexual reproduction, and metabolic adaptation lacking, however, reports of mushroom forming fungi so far. In Schizophyllum commune, an inositol monophosphatase has been found up-regulated during sexual development. The enzyme is crucial for inositol cycling where it catalyzes the last step of inositol phosphate metabolism restoring the inositol pool from the monophosphorylated inositol monophosphate. We overexpressed the gene in this model basidiomycete and verified its involvement in cell wall integrity and intracellular trafficking. Strong phenotypes in mushroom formation and cell metabolism were evidenced by proteome analyses. In addition, altered inositol signaling was shown to be involved in tolerance towards cesium and zinc, and increased metal tolerance towards cadmium, associated with induced expression of kinases and repression of phosphatases within the inositol cycle. The presence of the heavy metals Sr, Cs, Cd, and Zn lowered intracellular calcium levels. We could develop a model integrating inositol signaling in the known signal transduction pathways governed by Ras, G-protein coupled receptors, cAMP and elucidate different roles in development. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:52:24.680.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Krüger |
SpeciesList | scientific name: Schizophyllum commune H4-8; NCBI TaxID: 578458; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-03 08:03:02 | ID requested | |
1 | 2021-07-01 04:35:24 | announced | |
⏵ 2 | 2023-11-14 08:52:25 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: inositol monophosphatase, cell wall integrity, intracellular trafficking, heavy metals, sexual development,inositol phosphate signaling |
Contact List
Axel A. Brakhage |
contact affiliation | Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute Jena, Germany |
contact email | axel.brakhage@leibniz-hki.de |
lab head | |
Thomas Krüger |
contact affiliation | Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute |
contact email | thomas.krueger@leibniz-hki.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026465
- Label: PRIDE project
- Name: Inositol signaling in the basidiomycete fungus Schizophyllum commune