PXD026450 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SARS-CoV-2 spike protein glycosylation enriched by HILIC and Ti-IMAC |
Description | In this work, we performed a fully descriptive analysis N- and O- linked glycosylation of SARS-COV-2 S glycoprotein. We investigated that dual-functionalized Ti-IMAC material enable the simultaneous enrichment and separation of neutral and sialyl glycopeptides of a recombinant SARS-CoV-2 S glycoprotein from HEK293, which will eliminate the signal suppression of neutral glycopeptides to sialyl glycopeptides and improve the glycoform coverage of S protein. We have profiled 19 of its 22 potential N-glycosylated sites with 398 unique glycoforms in dual-functional Ti-IMIAC approach that is 1.6-fold of that in conventional HILIC method. We also identified O-linked glycosylation site that was not found in dual-functional Ti-IMIAC approach. In addition, we have also identified mannose-6-phosphate (M6P) glycosylation, which substantially expands the current knowledge of the spike protein’s glycosylation and enables the investigation of the influence of mannose-6-Phosphate on its cell entry. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:52:39.475.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Junfeng Huang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-03 02:43:26 | ID requested | |
1 | 2022-02-18 08:51:53 | announced | |
⏵ 2 | 2023-11-14 08:52:40 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Sars-cov-2, Covid-19 |
submitter keyword: SARS-CoV-2, Spike protein |
Contact List
Lingjun Li |
contact affiliation | 1School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53705, USA. Email: lingjun.li@wisc.edu 2Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53705, USA. |
contact email | lingjun.li@wisc.edu |
lab head | |
Junfeng Huang |
contact affiliation | University of Wisconsin-Madison |
contact email | jhuang373@wisc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD026450
- Label: PRIDE project
- Name: SARS-CoV-2 spike protein glycosylation enriched by HILIC and Ti-IMAC