PXD026378

PXD026378 is an original dataset announced via ProteomeXchange.

Title	Variation in upstream open reading frames contributes to allelic diversity in protein abundance
Description	The 5' untranslated region (UTR) sequence of eukaryotic mRNAs may contain upstream open reading frames (uORFs), which can regulate translation of the main open reading frame (mORF). The current model of translational regulation by uORFs posits that when a ribosome scans an mRNA and encounters a uORF, translation of that uORF can prevent ribosomes from reaching the mORF and cause decreased mORF translation. In this study, we first observed that rare variants in the 5' UTR dysregulate protein abundance. Upon further investigation, we found that rare variants near the start codon of uORFs can repress or derepress mORF translation, causing allelic changes in protein abundance. This finding holds for common variants as well, and common variants that modify uORF start codons also contribute disproportionately to metabolic and whole-plant phenotypes, suggesting that translational regulation by uORFs serves an adaptive function. These results provide evidence for the mechanisms by which natural sequence variation modulates gene expression, and ultimately, phenotype.
HostingRepository	PRIDE
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-02_04:11:25.345.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sujina Mali
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-31 07:12:36	ID requested
⏵ 1	2021-11-02 04:11:25	announced

Dataset with its publication pending

submitter keyword: Maize, proteomics, LC-MS, rare alleles, UTR, SNP

Richard D Vierstra
contact affiliation	Biology department, Washington University in Saint Louis, Saint Louis, MO, USA
contact email	rdvierstra@wustl.edu
lab head
Sujina Mali
contact affiliation	Washington University in St. Louis
contact email	sujinamali@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026378
     1. Label: PRIDE project
     2. Name: Variation in upstream open reading frames contributes to allelic diversity in protein abundance