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PXD026356

PXD026356 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDynamic FMR1 granule phase switch instructed by m6A modification contributes to maternal RNA decay
DescriptionTo search for the functional “m6A readers” specifically in the context of Drosophila early embryos, we incubated m6A-modified or unmodified RNA probes with cytosolic lysates from early embryos, and performed immunoprecipitation experiments followed by mass spectrometry analysis. We found that Drosophila FMRP (FMR1) was highly abundant in complexes immuno-precipitated by the m6A-modified probe. We performed data-independent acquisition (DIA) quantitative proteomics on the wild type and fmr1 maternal mutant embryos at multiple developmental stages, including 0-1 h, 2-3 h, and 5-6 h stages. To screen the FMR1-associated partners, we collected the 2–3-hour embryos and performed immunoprecipitation using the anti-Drosophila FMR1 antibody, followed by mass spectrometric analysis.
HostingRepositoryiProX
AnnounceDate2021-12-19
AnnouncementXMLSubmission_2021-12-19_00:29:31.903.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGuoqiang Zhang
SpeciesList scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-28 19:08:19ID requested
12021-12-19 00:29:32announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: drosophila,embryo,m6A, fmr1
Contact List
Dahua Chen
contact affiliationInstitute of Biomedical Research, Yunnan University
contact emailchendh@ioz.ac.cn
lab head
Guoqiang Zhang
contact affiliationInstitute of Biomedical Research, Yunnan University
contact emailgqzhang@ynu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI