PXD026356
PXD026356 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dynamic FMR1 granule phase switch instructed by m6A modification contributes to maternal RNA decay |
Description | To search for the functional “m6A readers” specifically in the context of Drosophila early embryos, we incubated m6A-modified or unmodified RNA probes with cytosolic lysates from early embryos, and performed immunoprecipitation experiments followed by mass spectrometry analysis. We found that Drosophila FMRP (FMR1) was highly abundant in complexes immuno-precipitated by the m6A-modified probe. We performed data-independent acquisition (DIA) quantitative proteomics on the wild type and fmr1 maternal mutant embryos at multiple developmental stages, including 0-1 h, 2-3 h, and 5-6 h stages. To screen the FMR1-associated partners, we collected the 2–3-hour embryos and performed immunoprecipitation using the anti-Drosophila FMR1 antibody, followed by mass spectrometric analysis. |
HostingRepository | iProX |
AnnounceDate | 2021-12-19 |
AnnouncementXML | Submission_2021-12-19_00:29:31.903.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Guoqiang Zhang |
SpeciesList | scientific name: Drosophila melanogaster; NCBI TaxID: 7227; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-05-28 19:08:19 | ID requested | |
⏵ 1 | 2021-12-19 00:29:32 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: drosophila,embryo,m6A, fmr1 |
Contact List
Dahua Chen | |
---|---|
contact affiliation | Institute of Biomedical Research, Yunnan University |
contact email | chendh@ioz.ac.cn |
lab head | |
Guoqiang Zhang | |
contact affiliation | Institute of Biomedical Research, Yunnan University |
contact email | gqzhang@ynu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |