PXD026343 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tensin3 interaction with talin drives formation of fibronectin-associated fibrillar adhesions |
Description | The formation of healthy tissue involves continuous remodelling of the extracellular matrix (ECM). Whilst it is known that this requires integrin-associated cell-ECM adhesion sites (CMAs) and actomyosin-mediated forces, the underlying mechanisms remain unclear. Here we examine how tensin3 contributes to formation of fibrillar adhesions (FBs) and fibronectin fibrillogenesis. Using BioID mass spectrometry and a mitochondrial targeting assay, we establish that tensin3 associates with the mechanosensors talin and vinculin. We show that the talin R11 rod domain binds directly to a helical motif within the central intrinsically disordered region (IDR) of tensin3, whilst vinculin binds indirectly to tensin3 via talin. Using CRISPR knock-out cells in combination with defined tensin3 mutations, we show (i) that tensin3 is critical for formation of alpha5 beta1-integrin FBs and for fibronectin fibrillogenesis, and (ii) the talin/tensin3 interaction drives this process, with vinculin acting to potentiate it. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:53:00.934.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Suat Peng Neo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-28 02:48:38 | ID requested | |
1 | 2022-09-09 03:11:16 | announced | |
⏵ 2 | 2023-11-14 08:53:01 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: vinculin, fibronectin,tensin, talin, extracellular matrix |
Contact List
Jayantha Gunaratne |
contact affiliation | Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, TBP Lab |
contact email | jayanthag@imcb.a-star.edu.sg |
lab head | |
Suat Peng Neo |
contact affiliation | Institute of Molecular and Cell Biology (ASTAR) |
contact email | neosp@imcb.a-star.edu.sg |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026343
- Label: PRIDE project
- Name: Tensin3 interaction with talin drives formation of fibronectin-associated fibrillar adhesions