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PXD026232
PXD026232 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Epigenetic regulation of nuclear lamina-associated heterochromatin domains by HAT1 and the acetylation of newly synthesized histones |
| Description | When DNA is replicated in eukaryotic cells, critical regulatory information conveyed by chemical modifications to DNA bases, histone proteins, and by the 3D architecture of the genome must be faithfully transmitted from parental to daughter cells. During DNA replication, parental nucleosomes are disrupted and re-deposited on the nascent DNA near their original location to preserve the spatial memory of the epigenetic modifications. Newly synthesized histones must be also incorporated into the nascent chromatin to maintain nucleosome density. Transfer of modification patterns from parental histones to new histones is a fundamental step in epigenetic inheritance. New histones have been thought to play a passive role in this process. Here we report that HAT1, which acetylates lysines 5 and 12 of newly synthesized histone H4 during replication-coupled chromatin assembly, regulates the epigenetic inheritance of chromatin states. HAT1 regulates the accessibility of large domains of heterochromatin termed HAT1-dependent Accessibility Domains (HADs). HADs are mega base-scale domains that comprise ~10% of the mouse genome. HADs display a high degree of overlap with a subset of Lamin-Associated Domains (LADs). HAT1 is required to maintain nuclear structure and integrity and physically associates with the nuclear lamina. HAT1 functions as a global negative regulator of H3 K9me2/3 and HADs correspond to the regions of the genome that have the highest density of H3 K9me3 peaks. These results indicate that HAT1 and the acetylation of newly synthesized histones are critical regulators of the epigenetic inheritance of heterochromatin and suggest a new mechanism for the epigenetic regulation of nuclear lamina-heterochromatin interactions. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-03 |
| AnnouncementXML | Submission_2026-04-03_09:23:49.951.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Dr. Miranda Gardner |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; N6-acetyl-L-lysine |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-05-24 16:47:16 | ID requested | |
| ⏵ 1 | 2026-04-03 09:23:50 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Histone Acetyltransferase1 (HAT1), Epigenetics, Heterochromatin, Nuclear Lamina, APEX2 Biotinylation, Bottom-Up Proteomics, Orbitrap Fusion |
Contact List
| Dr. Mark R. Parthun | |
|---|---|
| contact affiliation | The Ohio State University |
| contact email | parthun.1@osu.edu |
| lab head | |
| Dr. Miranda Gardner | |
| contact affiliation | The Ohio State University |
| contact email | gardner.207@osu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v03/MSV000087497/ |
