PXD025934 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Detecting mistranslation in yeast expressing tRNA variants |
Description | Mistranslation, the mis-incorporation of an amino acid not specified by the “standard” genetic code, occurs in all cells. tRNA variants that increase mistranslation arise spontaneously and engineered tRNAs can achieve mistranslation frequencies approaching 10% in yeast and bacteria. The goal of this study was to detect mistranslation from two different tRNA variants. The first variant, tRNA-Pro-G3:U70, has a mutation in its acceptor stem creating a G3:U70 base pair which is the key identity element for the alanine tRNA synthetase. This tRNA should be charged with alanine and mis-incorporate alanine at proline codons. The second variant, tRNA-Ser-UCU,G26A, is a serine tRNA with an arginine anticodon and a G26A secondary mutation to dampen function and prevent lethal levels of mistranslation. This tRNA should mis-incorporate serine at arginine codons. |
HostingRepository | PRIDE |
AnnounceDate | 2021-05-17 |
AnnouncementXML | Submission_2021-05-16_22:58:35.957.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matthew Berg |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-11 02:39:23 | ID requested | |
⏵ 1 | 2021-05-16 22:58:36 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Mistranslation, tRNA variants, yeast, LC-MSMS |
Contact List
Dr. Judit Villen |
contact affiliation | Department of Genome Science, University of Washington, USA |
contact email | jvillen@uw.edu |
lab head | |
Matthew Berg |
contact affiliation | Department of Genome Sciences, University of Washington |
contact email | mattberg@uw.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025934
- Label: PRIDE project
- Name: Detecting mistranslation in yeast expressing tRNA variants