PXD025869

PXD025869 is an original dataset announced via ProteomeXchange.

Title	Characterization of Simulium damnosum salivary antigens
Description	Simulium damnosum sensu lato (s.l.) blackflies transmit Onchocerca volvulus, a filarial nematode that causes human onchocerciasis. Human landing catches (HLCs) is currently the sole method used to estimate blackfly biting rates but is labour-intensive and questionable on ethical grounds. A potential alternative is to measure host antibodies to vector saliva deposited during bloodfeeding. In this study, the salivary proteome of S. damnosum s.l. was investigated. Blackflies were collected in Ghana by HLCs and dissected to extract their salivary glands. Salivary proteins were separated by gel-electrophoresis, and antigenic proteins visualized by immunoblot. Liquid chromatography mass spectrometry (LC–MS/MS) was performed to characterize the proteome of S. damnosum s.l. salivary glands. Several antigenic proteins were recognized, with the major ones located around 15 and 40 kDa. LC–MS/MS identified the presence of antigen 5-related protein, apyrase/nucleotidase, and hyaluronidase.
HostingRepository	PRIDE
AnnounceDate	2021-06-11
AnnouncementXML	Submission_2021-06-10_22:13:38.287.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025869
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Laura Willen
SpeciesList	scientific name: Simulium damnosum s.l. Me1; NCBI TaxID: 327042;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-07 04:21:41	ID requested
⏵ 1	2021-06-10 22:13:38	announced

Dataset with its publication pending

submitter keyword: Simulium damnosum s.l.

blackfly

saliva

antigens

exposure marker

Petr Volf
contact affiliation	Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
contact email	volf@cesnet.cz
lab head
Laura Willen
contact affiliation	Centre for the Evaluation of Vaccination, Vaccine & Infectious Diseases Institute, University of Antwerp
contact email	laura.willen@uantwerpen.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD025869

PRIDE project URI

• PRIDE
  1. PXD025869
     1. Label: PRIDE project
     2. Name: Characterization of Simulium damnosum salivary antigens