PXD025767

PXD025767 is an original dataset announced via ProteomeXchange.

Title	A quantitative map of human primary microRNA processing sites
Description	Maturation of canonical microRNA (miRNA) is initiated by DROSHA that cleaves the primary transcript (pri-miRNA). Over 1,800 miRNA loci are annotated in humans, but it remains largely unknown if and at which sites the pri-miRNAs are cleaved by DROSHA. Here we performed in vitro processing on a full set of human pri-miRNAs (miRBase v21) followed by sequencing. This comprehensive profiling enabled us to classify miRNAs based on DROSHA-dependence and map their cleavage sites with respective processing efficiency measures. Only 758 pri-miRNAs are confidently processed by DROSHA, while the majority may be non-canonical or false entries. Analyses of the DROSHA-dependent pri-miRNAs show key cis-elements for processing. We observe widespread alternative processing as well as unproductive cleavage events such as “nick” or “inverse” processing. SRSF3 is a broad-acting auxiliary factor modulating alternative processing and suppressing unproductive processing. The profiling data and methods developed in this study will allow systematic analyses of miRNA regulation.
HostingRepository	PRIDE
AnnounceDate	2021-07-28
AnnouncementXML	Submission_2021-07-27_21:57:47.658.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kijun Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-03 22:54:51	ID requested
⏵ 1	2021-07-27 21:57:48	announced

Dataset with its publication pending

submitter keyword: MicroRNA

Pri-miRNA processing

DROSHA

DGCR8

Microprocessor

SRSF3

RNA pull-down assay

V. Narry Kim
contact affiliation	Seoul National University
contact email	narrykim@snu.ac.kr
lab head
Kijun Kim
contact affiliation	Seoul National University
contact email	wonderful@snu.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025767

PRIDE project URI

• PRIDE
  1. PXD025767
     1. Label: PRIDE project
     2. Name: A quantitative map of human primary microRNA processing sites