PXD025732

PXD025732 is an original dataset announced via ProteomeXchange.

Title	Absolute proteome quantification in the gas-fermenting acetogen Clostridium autoethanogenum
Description	Microbes that can recycle one-carbon (C1) greenhouse gases into fuels and chemicals are vital for the biosustainability of future industries. Acetogens are the most efficient known microbes for fixing carbon oxides CO2 and CO. Understanding proteome allocation is important for metabolic engineering as it dictates metabolic fitness. Here, we use absolute proteomics to quantify intracellular concentrations for >1,000 proteins in the model-acetogen Clostridium autoethanogenum grown on three gas mixtures. We detect prioritisation of proteome allocation for C1 fixation and significant expression of proteins involved in the production of acetate and ethanol as well as proteins with unclear functions. The data also revealed which isoenzymes are important. Integration of proteomic and metabolic flux data demonstrated that enzymes catalyse high fluxes with high concentrations and high in vivo catalytic rates. We show that flux throughput was dominantly controlled through enzyme catalytic rates rather than concentrations. Our work serves as a reference dataset and advances systems-level understanding and engineering of acetogens.
HostingRepository	PRIDE
AnnounceDate	2022-05-27
AnnouncementXML	Submission_2022-05-27_09:36:12.888.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kaspar Valgepea
SpeciesList	scientific name: Clostridium autoethanogenum; NCBI TaxID: 84023;
ModificationList	6x(13)C; 6x(13)C; iodoacetamide derivatized amino-terminal residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-30 02:30:51	ID requested
⏵ 1	2022-05-27 09:36:13	announced

Dataset with its publication pending

submitter keyword: absolute quantification

acetogen

gas fermentation

data-independent acquisition

DIA

Esteban Marcellin
contact affiliation	Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Australia
contact email	e.marcellin@uq.edu.au
lab head
Kaspar Valgepea
contact affiliation	Australian Institute for Bioengineering and Nanotechnology,The University of Queensland
contact email	k.valgepea@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD025732

PRIDE project URI

• PRIDE
  1. PXD025732
     1. Label: PRIDE project
     2. Name: Absolute proteome quantification in the gas-fermenting acetogen Clostridium autoethanogenum