PXD025652

PXD025652 is an original dataset announced via ProteomeXchange.

Title	Evaluation of fully functionalized diazirine tags for chemical proteomic applications
Description	The use of photo-affinity labeling (PAL) reagents for the mapping of noncovalent small molecule-protein interactions has become widespread. Recently, several ‘fully-functionalized’ (FF) chemical tags have been developed wherein a photoactivatable capture group, an enrichment handle, and a functional group for synthetic conjugation to a molecule of interest are integrated into a single modular tag. Diazirine-based FF tags in particular are increasingly employed in chemical proteomic investigations, however, despite routine usage, their relative utility has not been established. Here, we systematically evaluate several diazirine-containing FF tags, including a terminal diazirine analog developed herein, for chemical proteomic investigations. Specifically, we compared the general reactivity of five diazirine tags and assessed their impact on the profiles of various small molecules, including fragments and known inhibitors revealing that such tags can have profound effects on the proteomic profiles of chemical probes. Our findings should be informative for chemical probe design, PAL-reagent development, and chemical proteomic investigations.
HostingRepository	PRIDE
AnnounceDate	2021-05-11
AnnouncementXML	Submission_2021-05-10_22:16:54.592.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louis Conway
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-27 08:18:33	ID requested
⏵ 1	2021-05-10 22:16:54	announced

Dataset with its publication pending

submitter keyword: photoaffinity, fully-functionlalized, diazirine

Christopher G Parker
contact affiliation	Department of Chemistry The Scripps Research Institute Jupiter FL, USA
contact email	cparker@scripps.edu
lab head
Louis Conway
contact affiliation	Scripps Research
contact email	lconway@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD025652

PRIDE project URI

• PRIDE
  1. PXD025652
     1. Label: PRIDE project
     2. Name: Evaluation of fully functionalized diazirine tags for chemical proteomic applications