PXD025618 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Disruption of nucleocytoplasmic trafficking as a cellular senescence driver |
Description | Senescent cells exhibit a reduced response to intrinsic and extrinsic stimuli. This reduction could be explained by disrupted nuclear transmission of signals. However, this hypothesis required more evidence to complete as a new modality of cellular senescence. Proteomic analysis of the cytoplasmic and nuclear fractions from young and senescent cells revealed disruption of nucleocytoplasmic trafficking (NCT) as an essential feature of replicative senescence (RS) at the global level. Blocking NCT either chemically or genetically induced RS-like senescence phenotypes, named as nuclear barrier-induced senescence (NBIS). Transcriptomic analysis revealed that NBIS had the most similar gene expression pattern to RS, compared with other stress-induced types of cellular senescence. Core proteomic and transcriptomic shared patterns between RS and NBIS included upregulation of endocytosis-lysosome network and downregulation of NCT in senescent cells, which were also conserved in yeast aging model. These results implicate an aging-dependent coordinated reduction in the transmission of extrinsic signals to the nucleus and in the nucleus-to-cytoplasm supply of proteins/RNAs. We further showed that the aging-associated decrease in Sp1 transcription factor expression was responsible for downregulation of NCT. Our results suggest that NBIS is a modality of cellular senescence that can represent the nature of physiological aging in eukaryotes. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-18 |
AnnouncementXML | Submission_2021-06-17_19:43:30.243.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD025618 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ji-Hwan Park |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | carbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-25 22:11:10 | ID requested | |
⏵ 1 | 2021-06-17 19:43:30 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: cellular senescence, replicative senescence, nucleocytoplasmic trafficking, nuclear barrier, eukaryotic aging |
Contact List
Daehee Hwang |
contact affiliation | Department of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea |
contact email | daehee@snu.ac.kr |
lab head | |
Ji-Hwan Park |
contact affiliation | Korea Bioinformation Center (KOBIC), Korea Research Institute of Bioscience and Biotechnology (KRIBB) |
contact email | kp706@postech.ac.kr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD025618
- Label: PRIDE project
- Name: Disruption of nucleocytoplasmic trafficking as a cellular senescence driver