PXD025598

PXD025598 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A three-organelle complex made by wrappER contacts with peroxisomes and mitochondria responds to liver lipid flux changes
Description	Hepatic lipid homeostasis depends on intracellular pathways that respire fatty acid in peroxisomes and mitochondria, and on systemic pathways that secrete fatty acid into the bloodstream, either free or condensed in very-low-density lipoprotein (VLDL) triglycerides. These systemic and intracellular pathways are interdependent, but it is unclear whether and how they integrate into a single cellular circuit. Here, we report that mouse liver wrappER, a distinct endoplasmic reticulum (ER) compartment with apparent fatty acid- and VLDL secretion functions, connects peroxisomes and mitochondria. Correlative light electron microscopy, quantitative serial section electron tomography and three-dimensional organelle reconstruction analysis show that the number of peroxisome-wrappER-mitochondria complexes changes throughout fasting-to-feeding transitions and doubles when VLDL synthesis stops following acute genetic ablation of Mttp in the liver. Quantitative proteomic analysis of peroxisome-wrappER-mitochondria complex-enriched fractions indicates that the loss of Mttp upregulates global fatty acid ?-oxidation, thereby integrating the dynamics of this three-organelle association into hepatic fatty acid flux responses. Therefore, liver lipid homeostasis occurs through the convergence of systemic and intracellular fatty acid-elimination pathways in the peroxisome-wrappER-mitochondria complex.
HostingRepository	MassIVE
AnnounceDate	2021-10-13
AnnouncementXML	Submission_2021-10-13_13:03:12.627.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luca Pellegrini
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-23 06:52:20	ID requested
⏵ 1	2021-10-13 13:03:13	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: WrappER, mitochondria, peroxisome, inter-organelle contact, fatty acid, beta-oxidation, very-low-density lipoprotein, Mttp

submitter keyword: WrappER, mitochondria, peroxisome, inter-organelle contact, fatty acid, beta-oxidation, very-low-density lipoprotein, Mttp

Contact List

Luca Pellegrini
contact affiliation	Laval University
contact email	luca.pellegrini@fmed.ulaval.ca
lab head
Luca Pellegrini
contact affiliation	Laval university
contact email	luca.pellegrini@fmed.ulaval.ca
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087291/