PXD025383 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The chimeric GaaR::XlnR transcription factor induces pectinolytic activities in the presence of D-xylose in Aspergillus niger |
Description | Aspergillus niger is a filamentous fungus well known for its ability to produce a wide variety of pectinolytic enzymes, which have many applications in the industry. The transcriptional activator GaaR is induced by 2-keto-3-deoxy-L-galactonate, a compound derived from D-galacturonic acid, and plays a major role in the regulation of pectinolytic genes. The requirement for inducer molecules can be a limiting factor for the production of enzymes. Therefore, the generation of chimeric transcription factors able to activate the expression of pectinolytic genes by using underutilized agricultural residues would be highly valuable for industrial applications. In this study, we used the CRISPR/Cas9 system to generate three chimeric GaaR::XlnR transcription factors by swapping the N-terminal region of the xylanolytic regulator XlnR to that of the GaaR in A. niger. As a test case, we constructed a PpgaX:hph reporter strain to evaluate the alteration of transcription factor specificity in the chimeric mutants. Additionally, we generated a constitutively active GaaR::XlnR V756F version of the most efficient chimeric transcription factor to better assess its activity. Our results showed that the chimeric GaaR::XlnR transcription factor was no longer induced in the presence of D-galacturonic acid, but in the presence of D-xylose instead. Moreover, proteomics analysis and saccharification assays confirmed the production of enzymes involved in the release of L-arabinose from pectin, while the constitutive version of this chimeric transcription factor showed consistently improved D-galacturonic acid release from pectin in a gaaR deletion background. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-18 |
AnnouncementXML | Submission_2021-11-18_06:50:37.590.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD025383 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Marcos Di Falco |
SpeciesList | scientific name: Aspergillus niger NRRL3; NCBI TaxID: 1451139; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-14 01:56:27 | ID requested | |
⏵ 1 | 2021-11-18 06:50:37 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Aspergillus niger, chimeric transcription factor, CRISPR/Cas9, pectinases, D-xylose |
Contact List
Ronald P. deVries |
contact affiliation | Westerdijik Fungal Biodiversity Institute Uppsalalaan 8, 3584 CT Utrecht, The Netherlands |
contact email | r.devries@wiknaw.nl |
lab head | |
Marcos Di Falco |
contact affiliation | Concordia University |
contact email | marcos.difalco@concordia.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025383
- Label: PRIDE project
- Name: The chimeric GaaR::XlnR transcription factor induces pectinolytic activities in the presence of D-xylose in Aspergillus niger