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PXD025356

PXD025356 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSecretory autophagy mediates mitochondria clearance in the absence of the mATG8-conjugation machinery
DescriptionPINK1-Parkin mediated mitophagy, a selective form of autophagy, represents an important mechanism in mitochondrial quality control (MQC) via clearance of damaged mitochondria. Although it is well known that the conjugation of mammalian ATG8s to phosphatidylethanolamine (PE) is a key step in autophagy, its role in mitophagy remains controversial. In this study, we attempted to clarify the role of the mATG8-conjugation system in mitophagy by generating knockouts of the mATG8-conjugation machinery. Unexpectedly, we show that mitochondria could still be cleared in the absence of mATG8 lipidation, in a process that was found to be independent of lysosomal degradation. Instead, mitochondria were found to be cleared via a secretory autophagy pathway, resulting in the extracellular release of mitochondria, in a process we defined as autophagic secretion of mitochondria (ASM). Functionally, increased ASM promoted the activation of the innate immune cGAS-STING pathway in recipient cells. Overall, data from this study reveal ASM as a novel mechanism in MQC, especially when the cellular mATG8-conjugation machinery is dysfunctional.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:39:19.995.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDennis Kappei
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList6x(13)C; 6x(13)C
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-12 23:28:54ID requested
12024-10-07 13:39:20announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mitochondria,Autophagy
Contact List
Dennis Kappei
contact affiliationCancer Science Institute of Singapore, National University of Singapore, Singapore
contact emaildennis.kappei@nus.edu.sg
lab head
Dennis Kappei
contact affiliationCancer Science Institute of Singapore
contact emaildennis.kappei@nus.edu.sg
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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