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PXD025344

PXD025344 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFruit fly O-GlcNAc glycoproteomics
DescriptionThe reversible posttranslational O-GlcNAc modification of serine or threonine residues of intracellular proteins is involved in many cellular events from signaling cascades to epigenetic and transcriptional regulation. O-GlcNAcylation is a conserved nutrient-dependent process involving two enzymes, O-GlcNAc Transferase (OGT) adding O-GlcNAc while O-GlcNAcase (OGA) removing it in a manner that’s protein and context dependent. O-GlcNAcylation is essential for epigenetic regulation of gene expression through its action on Polycomb and Trithorax and Compass complexes. However, the important role of O-GlcNAc on adult life and healthspan have been largely unexplored, mainly due the lack of available model systems. Cataloging the O-GlcNAc proteome has proven useful in understanding the biology of this modification in vivo. In this study, we leveraged a recently developed oga knockout fly mutant to identify the O-GlcNAcylated proteins in adult Drosophila melanogaster. The adult O-GlcNAc proteome revealed many proteins related to cell and organismal growth, development, differentiation, and epigenetics. We identified many O-GlcNAcylated proteins that serve a role in increased growth and decreased longevity, including HCF, SIN3A, LOLA, KISMET, ATX2, SHOT, and FOXO.
HostingRepositoryPRIDE
AnnounceDate2021-04-26
AnnouncementXMLSubmission_2021-04-26_06:36:36.730.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAdnan Halim
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListO-(N-acetylamino)glucosyl-L-threonine; O-(N-acetylamino)glucosyl-L-serine; monohydroxylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-12 22:51:46ID requested
12021-04-26 06:36:37announced
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP)
submitter keyword: Drosophila melanogaster, Fusion, O-GlcNAc
Contact List
Henrik Clausen
contact affiliationUniversity of Copenhagen Faculty of Health Sciences Centre for Glycomics Department of Cellular and Molecular Medicine Copenhagen Denmark
contact emailhclau@sund.ku.dk
lab head
Adnan Halim
contact affiliationUniversity of Copenhagen
contact emailhalim@sund.ku.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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