PXD025344 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Fruit fly O-GlcNAc glycoproteomics |
Description | The reversible posttranslational O-GlcNAc modification of serine or threonine residues of intracellular proteins is involved in many cellular events from signaling cascades to epigenetic and transcriptional regulation. O-GlcNAcylation is a conserved nutrient-dependent process involving two enzymes, O-GlcNAc Transferase (OGT) adding O-GlcNAc while O-GlcNAcase (OGA) removing it in a manner that’s protein and context dependent. O-GlcNAcylation is essential for epigenetic regulation of gene expression through its action on Polycomb and Trithorax and Compass complexes. However, the important role of O-GlcNAc on adult life and healthspan have been largely unexplored, mainly due the lack of available model systems. Cataloging the O-GlcNAc proteome has proven useful in understanding the biology of this modification in vivo. In this study, we leveraged a recently developed oga knockout fly mutant to identify the O-GlcNAcylated proteins in adult Drosophila melanogaster. The adult O-GlcNAc proteome revealed many proteins related to cell and organismal growth, development, differentiation, and epigenetics. We identified many O-GlcNAcylated proteins that serve a role in increased growth and decreased longevity, including HCF, SIN3A, LOLA, KISMET, ATX2, SHOT, and FOXO. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-26 |
AnnouncementXML | Submission_2021-04-26_06:36:36.730.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adnan Halim |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | O-(N-acetylamino)glucosyl-L-threonine; O-(N-acetylamino)glucosyl-L-serine; monohydroxylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-12 22:51:46 | ID requested | |
⏵ 1 | 2021-04-26 06:36:37 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP) |
submitter keyword: Drosophila melanogaster, Fusion, O-GlcNAc |
Contact List
Henrik Clausen |
contact affiliation | University of Copenhagen Faculty of Health Sciences Centre for Glycomics Department of Cellular and Molecular Medicine Copenhagen Denmark |
contact email | hclau@sund.ku.dk |
lab head | |
Adnan Halim |
contact affiliation | University of Copenhagen |
contact email | halim@sund.ku.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025344
- Label: PRIDE project
- Name: Fruit fly O-GlcNAc glycoproteomics