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PXD025304

PXD025304 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleElongation factor ELOF1 drives transcription-coupled repair and prevents genome instability
DescriptionCorrect transcription is crucial for life. However, DNA damage severely impedes elongating RNA Polymerase II (Pol II), causing transcription inhibition and transcription-replication conflicts. Cells are equipped with intricate mechanisms to counteract the severe consequence of these transcription-blocking lesions (TBLs). However, the exact mechanism and factors involved remain largely unknown. Here, using a genome-wide CRISPR/cas9 screen, we identified elongation factor ELOF1 as an important new factor in the transcription stress response upon DNA damage. We show that ELOF1 has an evolutionary conserved role in Transcription-Coupled Nucleotide Excision Repair (TC-NER), where it promotes recruitment of the TC-NER factors UVSSA and TFIIH to efficiently repair TBLs and resume transcription. Additionally, ELOF1 modulates transcription to protect cells from transcription-mediated replication stress, thereby preserving genome stability. Thus, ELOF1 protects the transcription machinery from DNA damage via two distinct mechanisms.
HostingRepositoryPRIDE
AnnounceDate2021-04-20
AnnouncementXMLSubmission_2021-04-20_04:46:47.455.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJeroen Demmers
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-09 06:16:33ID requested
12021-04-20 04:46:47announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Elongation factor, ELOF1, transcription-coupled DNA repair, genome instability
Contact List
Jeroen Demmers
contact affiliationProteomics Center | Erasmus University Medical Center | Rotterdam, the Netherlands
contact emailj.demmers@erasmusmc.nl
lab head
Jeroen Demmers
contact affiliationProteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands
contact emailj.demmers@erasmusmc.nl
dataset submitter
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Dataset FTP location
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