PXD025304 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Elongation factor ELOF1 drives transcription-coupled repair and prevents genome instability |
Description | Correct transcription is crucial for life. However, DNA damage severely impedes elongating RNA Polymerase II (Pol II), causing transcription inhibition and transcription-replication conflicts. Cells are equipped with intricate mechanisms to counteract the severe consequence of these transcription-blocking lesions (TBLs). However, the exact mechanism and factors involved remain largely unknown. Here, using a genome-wide CRISPR/cas9 screen, we identified elongation factor ELOF1 as an important new factor in the transcription stress response upon DNA damage. We show that ELOF1 has an evolutionary conserved role in Transcription-Coupled Nucleotide Excision Repair (TC-NER), where it promotes recruitment of the TC-NER factors UVSSA and TFIIH to efficiently repair TBLs and resume transcription. Additionally, ELOF1 modulates transcription to protect cells from transcription-mediated replication stress, thereby preserving genome stability. Thus, ELOF1 protects the transcription machinery from DNA damage via two distinct mechanisms. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-20 |
AnnouncementXML | Submission_2021-04-20_04:46:47.455.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jeroen Demmers |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-09 06:16:33 | ID requested | |
⏵ 1 | 2021-04-20 04:46:47 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Elongation factor, ELOF1, transcription-coupled DNA repair, genome instability |
Contact List
Jeroen Demmers |
contact affiliation | Proteomics Center | Erasmus University Medical Center | Rotterdam, the Netherlands |
contact email | j.demmers@erasmusmc.nl |
lab head | |
Jeroen Demmers |
contact affiliation | Proteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands |
contact email | j.demmers@erasmusmc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025304
- Label: PRIDE project
- Name: Elongation factor ELOF1 drives transcription-coupled repair and prevents genome instability