PXD025236

PXD025236 is an original dataset announced via ProteomeXchange.

Title	Temperature influences the composition and cytotoxicity of extracellular vesicles in Staphylococcus aureus
Description	Staphylococcus aureus is a pathogenic bacterium but also a commensal of skin and anterior nares in humans. As S. aureus transits from skins/nares to inside the human body, it experiences changes in temperature. The production and content of S. aureus extracellular vesicles (EV) have been increasingly studied over the past few years and EVs are increasingly being recognized as important to the infectious process. Nonetheless, the impact of temperature variation on S. aureus EVs has not been studied in detail as most reports that investigate EV cargoes and host cell interactions are performed using vesicles produced at 37˚C. Here we report that S. aureus vesicle production is temperature-dependent with greater vesiculation at 40˚C. We demonstrate that the temperature dependent regulation of vesicle production in S. aureus is mediated by the alpha phenol soluble modulin peptides (αPSMs). Through proteomic analysis, we observed increased packaging of virulence factors at 40˚C whereas more total proteins are present in EVs produced at 34˚C. Similar to the protein content, we perform transcriptomic analysis and demonstrate that the RNA cargo is impacted by temperature also. Finally, we demonstrate greater αPSM- and α-toxin mediated erythrocyte lysis with 40˚C EVs but 34˚C EVs are more cytotoxic toward THP-1 cells. Together our study demonstrates that small temperature variations have great impact on EV biogenesis and shape the interaction with host cells.
HostingRepository	PRIDE
AnnounceDate	2021-10-12
AnnouncementXML	Submission_2021-10-12_05:02:25.145.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ronan Carroll
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-06 19:48:32	ID requested
⏵ 1	2021-10-12 05:02:25	announced

Dataset with its publication pending

submitter keyword: Staphylococcus aureus EV vesicle temperature

Ronan Carroll
contact affiliation	Department of Biological Sciences, Ohio University
contact email	carrolr3@ohio.edu
lab head
Ronan Carroll
contact affiliation	Ohio University
contact email	carrolr3@ohio.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025236
     1. Label: PRIDE project
     2. Name: Temperature influences the composition and cytotoxicity of extracellular vesicles in Staphylococcus aureus