PXD025196

PXD025196 is an original dataset announced via ProteomeXchange.

Title	Embryonic developmental arrest in the annual killifish Austrolebias charrua: a proteomic approach to diapause III.
Description	Diapause is a reversible developmental arrest faced by many organisms in harsh environments. Annual killifish present this mechanism in three possible stages of development. Killifish are freshwater teleosts from Africa and America that live in ephemeral ponds, which dry up in the dry season. The juvenile and adult populations die, and the embryos remain buried in the bottom mud until the next rainy season. Thus, species survival is entirely embryo-dependent, and they are perhaps the most remarkable extremophile organisms among vertebrates. The aim of the present study was to gather information about embryonic diapauses with the use of a “shotgun” proteomics approach in diapause III and prehatching Austrolebias charrua embryos. Our results provide insight into the molecular mechanisms of diapause III. We detected a diapause-dependent change in a large group of proteins involved in different functions, such as metabolic pathways and stress tolerance, as well as proteins related to DNA repair and epigenetic modifications. Furthermore, we observed a diapauseassociated switch in cytoskeletal proteins. This first glance into global protein expression differences between prehatching and diapause III could provide clues regarding the induction/maintenance of this developmental arrest in A. charrua embryos. There appears to be no single mechanism underlying diapause and the present data expand our knowledge of the molecular basis of diapause regulation. This information will be useful for future comparative approaches among different diapauses in annual killifish and/or other organisms that experience developmental arrest.
HostingRepository	PRIDE
AnnounceDate	2021-05-12
AnnouncementXML	Submission_2021-05-11_22:50:16.249.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Analía Lima
SpeciesList	scientific name: Austrolebias; NCBI TaxID: 308056;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-06 04:02:08	ID requested
⏵ 1	2021-05-11 22:50:16	announced

Dataset with its publication pending

submitter keyword: Diapause III, annual killifish, development

María José Arezo
contact affiliation	Laboratorio de Biología Molecular de Organismos Acuáticos. Sección Biología Celular. Facultad de Ciencias. Universidad de la República. Montevideo - Uruguay
contact email	maui@fcien.edu.uy
lab head
Analía Lima
contact affiliation	Institut Pasteur de Montevideo, Proteomic and Analytical Biochemistry Unit
contact email	alima@pasteur.edu.uy
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD025196

PRIDE project URI

• PRIDE
  1. PXD025196
     1. Label: PRIDE project
     2. Name: Embryonic developmental arrest in the annual killifish Austrolebias charrua: a proteomic approach to diapause III.