PXD025146

PXD025146 is an original dataset announced via ProteomeXchange.

Title	The global Proteome and Ubiquitinome of bacterial and viral co-infected Bronchial Epithelial Cells
Description	Viral infections facilitate bacterial trafficking to the lower respiratory tract resulting in bacterial viral co infections. Bacterial dissemination to the lower respiratory tract is enhanced by influenza A virus induced epithelial cell damage and dysregulation of immune responses. Epithelial cells act as the first line of defense and detect pathogens by a high variety of pattern recognition receptors. The post translational modification ubiquitin is involved in almost every cellular process. Moreover, ubiquitination contributes to the regulation of host immune responses, influenza A virus uncoating and transport within host cells. We applied proteomics with a special focus on ubiquitination to assess the impact of single bacterial and viral as well as bacterial viral co-infections on bronchial epithelial cells. We used Tandem Ubiquitin Binding Entities to enrich polyubiquitinated proteins and assess changes in the ubiquitinome. Infecting 16HBE cells with Streptococcus pyogenes led to an increased abundance of proteins related to mitochondrial translation and energy metabolism in proteome and ubiquitinome. In contrast, influenza A virus infection mainly altered the ubiquitinome. Co-infections had no additional impact on protein abundances or affected pathways. Changes in protein abundance and enriched pathways were assigned to imprints of both infecting pathogens.
HostingRepository	PRIDE
AnnounceDate	2022-02-24
AnnouncementXML	Submission_2022-02-24_15:05:32.447.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Sura
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-01 03:34:46	ID requested
⏵ 1	2022-02-24 15:05:32	announced

Dataset with its publication pending

submitter keyword: co infection, ubiquitin, influenza A virus, Streptococcus pyogenes, Staphylococcus aureus, 16HBE, proteomics

Doerte Becher
contact affiliation	University of Greifswald, Center for Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics, Felix Hausdorff Str. 8, 17489 Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Thomas Sura
contact affiliation	University of Greifswald, Center for Functional Genomics of Microbes, Institute for Microbiology, Department of Microbial Proteomics
contact email	thomas.sura@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025146

PRIDE project URI

• PRIDE
  1. PXD025146
     1. Label: PRIDE project
     2. Name: The global Proteome and Ubiquitinome of bacterial and viral co-infected Bronchial Epithelial Cells