PXD024949

PXD024949 is an original dataset announced via ProteomeXchange.

Title	Fast and global reorganization of the chloroplast protein biogenesis network during heat acclimation
Description	Heat waves caused by global warming severely challenge photosynthesis and hence plant biomass production, but the mechanisms of its adjustment, repair and biogenesis during heat acclimation are elusive. We provide a global and time-resolved insight, how these processes are modulated during heat acclimation in chloroplasts, revealing profound modulation of protein biogenesis. Ribosome profiling with Chlamydomonas reinhardtii showed early translational downregulation during synthesis of photosynthesis core antenna proteins, a response that is conserved in Nicotiana tabacum. Our data suggest that these early effects may be caused by reduced translocation of ribosomes synthesizing thylakoid proteins, and that later heat acclimation alleviates these effects. Analysis of the proteinaceous interactome of plastid ribosomes demonstrates widespread remodeling of ribosome-associated processes that could be responsible for the early translational impacts and subsequent acclimation responses. The role of chlorophyll metabolism, protein targeting, and assembly of photosystem complexes as co-translational bottlenecks during heat acclimation are discussed.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:46.701.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Räschle
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-23 06:27:10	ID requested
1	2022-01-06 00:50:15	announced
⏵ 2	2023-11-14 08:52:47	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: photosynthesis biogenesis, protein complex assembly, translational regulation, protein synthesis, heat acclimation,Chloroplast

Zuzana Storchova
contact affiliation	Molecular Genetics TU Kaiserslautern Germany
contact email	storchova@bio.uni-kl.de
lab head
Markus Räschle
contact affiliation	Technical University Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD024949

PRIDE project URI

• PRIDE
  1. PXD024949
     1. Label: PRIDE project
     2. Name: Fast and global reorganization of the chloroplast protein biogenesis network during heat acclimation