PXD024933 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Examining pathways of iron and sulfur acquisition, trafficking, deployment, and storage in mineral-grown methanogen cells |
Description | Methanogens were recently shown to reduce pyrite (FeS2) generating aqueous iron-sulfide (FeS(aq)) clusters that are likely assimilated as a source of Fe and S. Here, we compare the phenotype of Methanococcus voltae when grown with FeS2 or ferrous iron (Fe(II)) and sulfide (HS-). Differential proteomic analyses showed similar expression of core methanogenesis enzymes, indicating that Fe and S source does not substantively alter the energy metabolism of cells. However, a homolog of the Fe(II) transporter FeoB and its transcriptional regulator DtxR were up-expressed in FeS2 grown cells, indicating that cells sense Fe(II) limitation. Two homologs of IssA, a protein putatively involved in coordinating thioferrate nanoparticles, were also up-expressed in FeS2 grown cells. We interpret these data to indicate that DtxR cannot sense Fe(II) and therefore cannot down-regulate FeoB. We suggest this is due to the transport of Fe(II) complexed with sulfide (FeS(aq)) leading to excess Fe that is sequestered by IssA as a thioferrate-like species. This model provides a framework for the design of targeted experiments aimed at further characterizing Fe acquisition and homeostasis in M. voltae and other methanogens. |
HostingRepository | PRIDE |
AnnounceDate | 2021-07-07 |
AnnouncementXML | Submission_2021-07-07_13:47:31.533.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rachel Spietz |
SpeciesList | scientific name: Methanococcus voltae A3; NCBI TaxID: 456320; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-23 00:21:28 | ID requested | |
⏵ 1 | 2021-07-07 13:47:31 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: methanogen, iron-sulfur cluster, FeS2, pyrite, mackinawite, FeS |
Contact List
Boyd, Eric S. |
contact affiliation | Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, 59717 |
contact email | eric.boyd@montana.edu |
lab head | |
Rachel Spietz |
contact affiliation | Montana State University |
contact email | rachel.spietz@montana.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024933
- Label: PRIDE project
- Name: Examining pathways of iron and sulfur acquisition, trafficking, deployment, and storage in mineral-grown methanogen cells