PXD024891 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic and lipidomic profiling of demyelinating lesions identifies fatty acids as modulators in inflammation resolution |
Description | After demyelinating injury of the central nervous system, resolution of the mounting acute innate inflammation is crucial for the initiation of a regenerative response. To identify factors in lesion recovery after demyelination injury, we used a toxin-induced model, in which a single dose of lysolecithin is injected into the corpus callosum to induce a focal demyelinating lesion. Afterwards, we investigated the proteome of demyelinating lesions at different time points post injection (dpi) in a time resolved manner. Lesion sites were excised analyzed from different mice at 0 dpi, without lysolecithin injection, as well as lysolecithin treated mice at 3 dpi, 7 dpi, and 14 dpi. Overall, immune cell migration, especially infiltration of microglia and macrophages, as well as fatty acid metabolism are playing crucial roles for the immidiate response, repair and finally lesion recovery. Additionally, Using lipidomics and eicosanoidomics, we identified bioactive lipids in the resolution phase of inflammation with a marked induction of n-3 polyunsaturated fatty acids. Using fat-1 transgenic mice, which convert n-6 fatty acids to n-3 fatty acids, we found that reduction of the n-6/n-3 ratio facilitates inflammation resolution. In addition, we observed accelerated inflammation resolution and enhanced generation of oligodendrocytes in aged mice when n-3 fatty acids are shuttled to the brain. Thus, n-3 fatty acids and eicosanoids, their oxidized bioactive products, enhance lesion recovery and may therefore provide the basis for novel pro-regenerative medicines of demyelinating diseases in the central nervous system. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-30 |
AnnouncementXML | Submission_2021-09-30_06:15:43.482.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stephan Mueller |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | amidated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-22 00:06:32 | ID requested | |
⏵ 1 | 2021-09-30 06:15:43 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Demyelination, Myelin, Microglia, Oligodendrocytes |
Contact List
Stefan F. Lichtenthaler |
contact affiliation | DZNE - German Center for Neurodegenerative Diseases Munich, Neuroproteomics, Feodor-Lynen Str. 17, D-81377 Munich, Germany |
contact email | stefan.lichtenthaler@dzne.de |
lab head | |
Stephan Mueller |
contact affiliation | DZNE Munich Neuroproteomics |
contact email | stephan.mueller@dzne.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD024891
- Label: PRIDE project
- Name: Proteomic and lipidomic profiling of demyelinating lesions identifies fatty acids as modulators in inflammation resolution