PXD024822

PXD024822 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The human GID complex engages two independent modules for substrate recruitment
Description	Chemical cross-linking coupled to mass spectrometry was used to study two different assemblies of the human E3 ubiquitin ligase complex, GID. The 5mer complex consisted of subunits MAEA, RMND5a, TWA1, WDR26, and RanBP9; the 6mer complex consisted of the subunits of the 5mer complex and the additional subunit ARMC8β. Cross-linking experiments were performed with disuccinimidyl suberate (DSS) or pimelic dihydrazide (PDH) in combination with the coupling reagent DMTMM.
HostingRepository	PRIDE
AnnounceDate	2021-08-25
AnnouncementXML	Submission_2021-08-25_03:56:33.008.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Leitner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-18 02:57:16	ID requested
⏵ 1	2021-08-25 03:56:33	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Chemical cross-linking
DSS
PDH
DMTMM
structural proteomics
GID complex

submitter keyword: Chemical cross-linking

DSS

PDH

DMTMM

structural proteomics

GID complex

Contact List

Alexander Leitner
contact affiliation	ETH Zurich, Department of Biology, Institute of Molecular Systems Biology
contact email	leitner@imsb.biol.ethz.ch
lab head
Alexander Leitner
contact affiliation	ETH Zurich, Institute of Molecular Systems Biology
contact email	leitner@imsb.biol.ethz.ch
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD024822
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD024822

PRIDE project URI

Repository Record List

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