PXD024816

PXD024816 is an original dataset announced via ProteomeXchange.

Title	Mutation of Arabidopsis SME1 improves oxidative stress resilience
Description	Alternative splicing is an important posttranscriptional gene regulatory process, acting in diverse adaptive and basal plant processes. Splicing of precursor-messenger RNA (pre-mRNA) is catalyzed by a dynamic ribonucleoprotein complex, termed the spliceosome. In a suppressor screen, we identified a nonsense mutation in the Sm protein SME1 to alleviate photorespiratory H2O2-dependent cell death in catalase-deficient plants. Furthermore, sme1-2 mutants showed increased tolerance to the superoxide-generating herbicide methyl viologen. mRNA-seq and proteomic analyses indicated a constitutive stress response and pre-mRNA splicing alterations in sme1-2 mutants under control conditions. With a pulldown experiment, using SME1 as a bait, we provide experimental evidence for proteins associated with Arabidopsis thaliana spliceosome complexes, by identifying almost 50 proteins homologous to mammalian spliceosome-associated proteins and propose four novel proteins previously not-reported or hypothesized to be part of plant spliceosome complexes. Mutation of one of these interactors, the Sm core assembly protein ICLN also resulted in a decreased sensitivity to methyl viologen. Taken together, we show that SME1 mutation and impaired snRNP assembly result in enhanced resilience to oxidative stress.
HostingRepository	PRIDE
AnnounceDate	2023-03-03
AnnouncementXML	Submission_2023-03-03_00:49:58.228.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PatrickWillems
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-18 01:02:37	ID requested
⏵ 1	2023-03-03 00:49:58	announced

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana, spliceosome, CATALASE2, oxidative stress, Sm protein

Frank VanBreusegem
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
contact email	frbre@psb.ugent.be
lab head
PatrickWillems
contact affiliation	PSB-VIB
contact email	pawil@psb.ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024816
     1. Label: PRIDE project
     2. Name: Mutation of Arabidopsis SME1 improves oxidative stress resilience